Searched for: department%3A%22ImPhys%255C%257Eslsh%257EImaging%255C+Physics%22
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Ossenkoppele, B.W. (author), Luijten, Ben (author), Bera, Deep (author), de Jong, N. (author), Verweij, M.A. (author), van Sloun, Ruud J.G. (author)
There is an increased desire for miniature ultrasound probes with small apertures to provide volumetric images at high frame rates for in-body applications. Satisfying these increased requirements makes simultaneous achievement of a good lateral resolution a challenge. As micro-beamforming is often employed to reduce data rate and cable count...
journal article 2023
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Haghparast, S. (author), Stallinga, S. (author), Rieger, B. (author)
Fusion of multiple chemically identical complexes, so-called particles, in localization microscopy, can improve the signal-to-noise ratio and overcome under-labeling. To this end, structural homogeneity of the data must be assumed. Biological heterogeneity, however, could be present in the data originating from distinct conformational...
journal article 2023
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van den Akker, H.E.A. (author), Mudde, R.F. (author)
De transport- en overdrachtsprocessen van warmte, massa en impuls worden naar hun analogie behandeld. De processen worden beschreven met behulp van macro- en microbalansen die in veel gevallen leiden tot differentiaalvergelijkingen. Ook veel aspecten van stromingsleer komen op deze manier aan de orde en zo bereidt het boek ook voor op moderne...
book 2023
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Wang, W. (author), Jakobi, A. (author), Wu, Yu-Le (author), Ries, Jonas (author), Stallinga, S. (author), Rieger, B. (author)
Single molecule localization microscopy offers resolution nearly down to the molecular level with specific molecular labelling, and is thereby a promising tool for structural biology. In practice, however, the actual value to this field is limited primarily by incomplete fluorescent labelling of the structure. This missing information can be...
journal article 2023
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Wang, W. (author), Jakobi, A. (author), Wu, Yu‑Le ‑L (author), Ries, Jonas (author), Stallinga, S. (author), Rieger, B. (author)
Correction to: Scientific Reports, published online 16 August 2023 The original version of this Article contained an error in the upper inset of Figure 4, where the atomic model was missing. The original Figure 4 and accompanying legend appear below. (Figure presented.) Overlay of the fluorophore positions from the SMLM particle fusion data ...
journal article 2023
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Schaeken, Teun (author), Hoogerbrugge, L.A. (author), Verschuur, D.J. (author)
The acoustic wave equation describes wave propagation directly from basic physical laws, even in heterogeneous acoustic media. When numerically simulating waves with the wave equation, contrasts in the medium parameters automatically generate all scattering effects. For some applications - such as propagation analysis or certain wave-equation...
journal article 2022
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Lane, R. (author), Wolters, Anouk H.G. (author), Giepmans, Ben N.G. (author), Hoogenboom, J.P. (author)
Volume electron microscopy (EM) of biological systems has grown exponentially in recent years due to innovative large-scale imaging approaches. As a standalone imaging method, however, large-scale EM typically has two major limitations: slow rates of acquisition and the difficulty to provide targeted biological information. We developed a 3D...
journal article 2022
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Hari, S. (author), Slotman, Johan A. (author), Vos, Y. (author), Floris, Christian (author), van Cappellen, W.A. (author), Hagen, C.W. (author), Stallinga, S. (author), Houtsmuller, A.B. (author), Hoogenboom, J.P. (author)
Super-resolution fluorescence microscopy can be achieved by image reconstruction after spatially patterned illumination or sequential photo-switching and read-out. Reconstruction algorithms and microscope performance are typically tested using simulated image data, due to a lack of strategies to pattern complex fluorescent patterns with...
journal article 2022
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Hopf, Y.M. (author), Ossenkoppele, B.W. (author), Noothout, E.C. (author), Chang, Z.Y. (author), Chen, C. (author), Vos, H.J. (author), Verweij, M.D. (author), de Jong, N. (author), Pertijs, M.A.P. (author)
This work describes an ASIC design for high-frame-rate 3D intracardiac echocardiography probes. The chip is the first to combine element-level high-voltage pulsers and time-gain-compensation analog frontends as well as subarray beamformers and in-probe digitization in a pitch-matched fashion. The integration challenge is met by a shared hybrid...
poster 2022
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Thorsen, R.Ø. (author), Hulleman, C.N. (author), Rieger, B. (author), Stallinga, S. (author)
Combining orientation estimation with localization microscopy opens up the possibility to analyze the underlying orientation of biomolecules on the nanometer scale. Inspired by the recent improvement of the localization precision by shifting excitation patterns (MINFLUX, SIMFLUX), we have adapted the idea towards the modulation of excitation...
journal article 2022
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Soozande, Mehdi (author), Ossenkoppele, B.W. (author), Hopf, Y.M. (author), Pertijs, M.A.P. (author), Verweij, M.D. (author), de Jong, N. (author), Vos, H.J. (author), Bosch, Johan G. (author)
Atrial fibrillation (AF) is the most common cardiac arrhythmia and is normally treated by RF ablation. Intracardiac echography (ICE) is widely employed during RF ablation procedures to guide the electrophysiologist in navigating the ablation catheter, although only 2-D probes are currently clinically used. A 3-D ICE catheter would not only...
journal article 2022
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Wang, W. (author), Heydarian, H. (author), Huijben, T.A.P.M. (author), Stallinga, S. (author), Rieger, B. (author)
Summary: We present a fast particle fusion method for particles imaged with single-molecule localization microscopy. The state-of-the-art approach based on all-to-all registration has proven to work well but its computational cost scales unfavorably with the number of particles N, namely as N2. Our method overcomes this problem and achieves a...
journal article 2022
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Kalisvaart, D. (author), Cnossen, J.P. (author), Hung, S. (author), Stallinga, S. (author), Verhaegen, M.H.G. (author), Smith, C.S. (author)
Modulation enhanced single-molecule localization microscopy (meSMLM) methods improve the localization precision by using patterned illumination to encode additional position information. Iterative meSMLM (imeSMLM) methods iteratively generate prior information on emitter positions, used to locally improve the localization precision during...
journal article 2022
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Siemons, Marijn E. (author), Kapitein, Lukas C. (author), Stallinga, S. (author)
Single-molecule localization microscopy has developed into a widely used technique to overcome the diffraction limit and enables 3D localization of single-emitters with nanometer precision. A widely used method to enable 3D encoding is to use a cylindrical lens or a phase mask to engineer the point spread function (PSF). The performance of...
journal article 2022
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Stallinga, S. (author), Radmacher, Niels (author), Delon, Antoine (author), Enderlein, Jörg (author)
One of the fundamental limits of classical optical microscopy is the diffraction limit of optical resolution. It results from the finite bandwidth of the optical transfer function (or OTF) of an optical microscope, which restricts the maximum spatial frequencies that are transmitted by a microscope. However, given the frequency support of the...
journal article 2022
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Ossenkoppele, B.W. (author), Wei, Luxi (author), Luijten, Ben (author), Vos, H.J. (author), de Jong, N. (author), Van Sloun, Ruud J.G. (author), Verweij, M.D. (author)
3-D contrast enhanced ultrasound enables better visualization of inherently 3-D vascular geometries compared to an intersecting plane. Additionally, it would allow the application of motion correction techniques for all directions. Both contrast detection and motion correction work better on high-frame rate data. However high-frame rate 3-D...
conference paper 2022
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Stevens, T.N.M. (author), Herbst, Elizabeth B. (author), Luijten, Ben (author), Ossenkoppele, B.W. (author), Voskuil, Thierry J. (author), Wang, Shiying (author), Youn, Jihwan (author), Errico, Claudia (author), Pezzotti, Nicola (author)
The image quality of ultrasound localization microscopy (ULM) images is driven by the ability to accurately detect and track the location of microbubbles (MBs) in vascular networks. This task becomes increasingly challenging in imaging environments with high MB concentrations and low signal-to-noise ratios, making it difficult to...
conference paper 2022
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Hinsdale, T.A. (author), Stallinga, S. (author), Rieger, B. (author)
Structured illumination microscopy (SIM) is a widely used imaging technique that doubles the effective resolution of widefield microscopes. Most current implementations rely on diffractive elements, either gratings or programmable devices, to generate structured light patterns in the sample. These can be limited by spectral efficiency, speed, or...
journal article 2021
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Hulleman, C.N. (author), Thorsen, R.Ø. (author), Kim, E. (author), Dekker, C. (author), Stallinga, S. (author), Rieger, B. (author)
Estimating the orientation and 3D position of rotationally constrained emitters with localization microscopy typically requires polarization splitting or a large engineered Point Spread Function (PSF). Here we utilize a compact modified PSF for single molecule emitter imaging to estimate simultaneously the 3D position, dipole orientation, and...
journal article 2021
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Huijben, T.A.P.M. (author), Heydarian, H. (author), Auer, Alexander (author), Schueder, Florian (author), Jungmann, Ralf (author), Stallinga, S. (author), Rieger, B. (author)
Particle fusion for single molecule localization microscopy improves signal-to-noise ratio and overcomes underlabeling, but ignores structural heterogeneity or conformational variability. We present a-priori knowledge-free unsupervised classification of structurally different particles employing the Bhattacharya cost function as dissimilarity...
journal article 2021
Searched for: department%3A%22ImPhys%255C%257Eslsh%257EImaging%255C+Physics%22
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