- document
-
Doloman, Anna (author), de Bruin, S. (author), van Loosdrecht, Mark C.M. (author), Sousa, Diana Z. (author), Lin, Y. (author)Glycans are crucial for the structure and function of anaerobic granular sludge in wastewater treatment. Yet, there is limited knowledge regarding the microorganisms and biosynthesis pathways responsible for glycan production. In this study, we analysed samples from anaerobic granular sludges treating papermill and brewery wastewater,...journal article 2024
- document
-
Szabo Corbacho, M. (author), Sharma, Pragnya (author), Míguez, Diana (author), de la Sovera, Victoria (author), Brdjanovic, Damir (author), Etchebehere, Claudia (author), Garcia, H. (author), van Lier, J.B. (author)The study investigated the effects of long-chain fatty acids (LCFA) on anaerobic sludge treating lipid-rich wastewater. It involved batch experiments with three sludge samples: two acclimated to lipids and one non-acclimated. The experiments aimed to observe the degradation of LCFA, specifically oleate and palmitate, by dosing them at...journal article 2024
- document
-
Haaksman, V.A. (author), van Dijk, E.J.H. (author), Al-Zuhairy, Salah (author), Mulders, Michel (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)The development of continuous flow reactors (CFRs) employing aerobic granular sludge (AGS) for the retrofit of existing wastewater treatment plants (WWTPs) using a continuous-flow activated sludge (CFAS) system has garnered increasing interest. This follows the worldwide adoption of AGS technology in sequencing batch reactors (SBRs). The...journal article 2024
- document
-
Garcia Rea, V.S. (author)Anaerobic digestion (AD) is a biochemical process in which organic matter is converted into biogas in a series of biochemical reactions. The development of high-rate anaerobic reactors (HRAR) led to the breakthrough of full-scale applications of AD for the treatment of industrial wastewater. HRARs, such as the upflow anaerobic sludge blanket ...doctoral thesis 2023
- document
-
Wasajja, H. (author)Biomass is predominantly the major source of energy in the global south. It is the readily available source of energy in global south and is used in rural energy households in the form of wood, charcoal and agricultural residues. However, biomass energy source is not utilised in the most efficient way and hence there is still a gap in achieving...doctoral thesis 2023
- document
-
van Aalst, A.C.A. (author)The production of ethanol by the yeast Saccharomyces cerevisiae remains the process in industrial biotechnology with the largest product volume (ca. 100 million litres annually in 2022). Carbon losses occur due to the formation of biomass and glycerol, which can account for at least 8% of the product. Under anaerobic conditions, formation of...doctoral thesis 2023
- document
-
Zha, Qinying (author)Driven by the increasing demand for waste reduction and green energy production, an integrated system which combines an anaerobic membrane bioreactor (AnMBR) and a solid oxide fuel cell (SOFC) was proposed in this research project for blackwater treatment and energy production. The potentials of using an AnMBR for wastewater treatment and biogas...master thesis 2023
- document
-
Chowdhary, Kshitij (author)Of all the greenhouse gases (GHGs), carbon dioxide (CO2) has been the target of most climate recovery efforts as it is the most abundantly emitted GHG by mass. In fact, in 2015 a legally binding international treaty was adopted by 196 parties in Paris, France to constrain the anthropogenic warming to 1.5-2.0˚C above the pre-industrial level. In...master thesis 2023
- document
-
Mostafa Mohamed Abdelsadek Elshourbagy, Mostafa (author)Aromatic compounds have always been of concern regarding their toxicity to living organisms, including microorganisms. With more anthropogenic activities (e.g. coal gasification), the need for feasible treatment of industrial effluents is highly prioritised. With the anaerobic degradation process being a competitive solution, these compounds’...master thesis 2023
- document
-
Verburg, Dylan (author)On the 14th of July 2013, a methane peak was observed in the Gale crater. This methane peak has been investigated in a non-biological context before. This study applies exoplanet research strategies and knowledge about biological methane production on Earth to investigate the biological context. Bayes formula is the foundation of the Bayesian...master thesis 2023
- document
-
Deng, Z. (author), Ferreira, Ana Lucia Morgado (author), Spanjers, H. (author), van Lier, J.B. (author)Bovine serum albumin (BSA) and casein (CAS) were used in batch tests to compare the protein degradation in the presence and absence of carbohydrates and volatile fatty acids (VFAs). The modified Gompertz model was applied to estimate reaction rates. The results showed that deamination was the rate-limiting step, with a rate ranging between 2...journal article 2023
- document
-
Pavez Jara, J.A. (author), van Lier, J.B. (author), de Kreuk, M.K. (author)Full-scale thermal hydrolysis processes (THP) showed an increase in nutrients release and formation of melanoidins, which are considered to negatively impact methanogenesis during mesophilic anaerobic digestion (AD). In this research, fractionation of THP-sludge was performed to elucidate the distribution of nutrients and the formed...journal article 2023
- document
-
Wei, P. (author), Uijttewaal, W.S.J. (author), Spanjers, H. (author), van Lier, J.B. (author), de Kreuk, M.K. (author)Gas-mixing is commonly applied in anaerobic digesters, yet the resulting flow and hydraulic mixing are difficult to evaluate because of limited full-scale experimental data and uncertainties in integrating sludge rheological data. This study used computational fluid dynamics (CFD) to assess the impact of treated sludge rheology on flow and...journal article 2023
- document
-
van Aalst, A.C.A. (author), Jansen, Mickel L.A. (author), Mans, R. (author), Pronk, J.T. (author)Background: Anaerobic Saccharomyces cerevisiae cultures require glycerol formation to re-oxidize NADH formed in biosynthetic processes. Introduction of the Calvin-cycle enzymes phosphoribulokinase (PRK) and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) has been shown to couple re-oxidation of biosynthetic NADH to ethanol...journal article 2023
- document
-
Haaksman, V.A. (author), Schouteren, M. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)There is a growing interest to implement aerobic granular sludge (AGS) in existing conventional activated sludge (CAS) systems with a continuous flow-through configuration. The mode of anaerobic contact of raw sewage with the sludge is an important aspect in the adaptation of CAS systems to accommodate AGS. It remains unclear how the...journal article 2023
- document
-
Cabeza, Camila (author), van Lier, J.B. (author), van der Steen, Peter (author)Microalgae-bacteria biomass was digested through anaerobic digestion (AD) to produce biogas. Since microalgae typically have a more resistant cell wall than activated sludge (AS), its anaerobic biodegradability is limited. Therefore, three different pre-treatment techniques were evaluated for their effect on anaerobic biodegradability,...journal article 2023
- document
-
Deng, Z. (author), Muñoz Sierra, Julian (author), Ferreira, Ana Lucia Morgado (author), Cerqueda Garcia, D. (author), Spanjers, H. (author), van Lier, J.B. (author)Treating protein-rich wastewater using cost-effective and simple-structured single-stage reactors presents several challenges. In this study, we applied an anaerobic sequencing batch reactor (AnSBR) to treat protein-rich wastewater from a slaughterhouse. We focused on identifying the key factors influencing the removal of chemical oxygen...journal article 2023
- document
-
Zander, F. (author), Comans, R.N.J. (author), Gebert, J. (author)Degradability of organic matter in river sediments differs in relation to origin and age. In order to explain previously observed spatial patterns of organic matter degradability and stabilization, this study investigated sediment organic matter (SOM) properties along a tidal Elbe river transect using dissolved organic matter (DOM) fractions,...journal article 2023
- document
-
Olavarria, Karel (author), Sousa, Diana Z. (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)Utilizing anaerobic metabolisms for the production of biotechnologically relevant products presents potential advantages, such as increased yields and reduced energy dissipation. However, lower energy dissipation may indicate that certain reactions are operating closer to their thermodynamic equilibrium. While stoichiometric analyses and...journal article 2023
- document
-
Hu, Zhi Yi (author), Lin, Yi Peng (author), Wang, Qing Ting (author), Zhang, Yi Xin (author), Tang, Jie (author), Wang, Shuai (author), van Loosdrecht, Mark C.M. (author), Zeng, Raymond Jianxiong (author), Zhang, Fang (author)By maintaining the cell integrity of waste activated sludge (WAS), structural extracellular polymeric substances (St-EPS) resist WAS anaerobic fermentation. This study investigates the occurrence of polygalacturonate in WAS St-EPS by combining chemical and metagenomic analyses that identify ∼22% of the bacteria, including Ferruginibacter and...journal article 2023