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Gomez de Santos, Patricia (author), González-Benjumea, Alejandro (author), Fernandez-Garcia, Angela (author), Aranda, Carmen (author), Wu, Y. (author), But, A. (author), Molina-Espeja, Patricia (author), Zhang, W. (author), Hollmann, F. (author)The hydroxylation of fatty acids is an appealing reaction in synthetic chemistry, although the lack of selective catalysts hampers its industrial implementation. In this study, we have engineered a highly regioselective fungal peroxygenase for the ω-1 hydroxylation of fatty acids with quenched stepwise over-oxidation. One single mutation near...journal article 2023
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Li, Z. (author), Liu, Haoyuan (author), Hicks, M.A. (author), Pisano, F. (author)Offshore monopile foundations are exposed to misaligned wind and wave loadings, which are respectively dominated by (nearly) static and cyclic load components. While the response of these systems to unidirectional cyclic loading has been extensively investigated, only a few studies have been devoted to the realistic case of misaligned static...journal article 2023
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Tsitsilonis, Konstantinos Marios (author), Theotokatos, Gerasimos (author), Patil, Chaitanya (author), Coraddu, A. (author)The advancements in digital twins when combined with the use of the machine learning tools can facilitate the effective health assessment and diagnostics of safety critical systems. This study aims at developing a framework to address the health assessment of marine engines utilising digital twins based on first-principles. This framework...journal article 2023
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Windt, L. M. (author), Wiendels, M. (author), Dostanić, M. (author), Bellin, M. (author), Sarro, Pasqualina M (author), Mastrangeli, Massimo (author), Mummery, C. L. (author), van Meer, B.J. (author)Human heart tissues grown as three-dimensional spheroids and consisting of different cardiac cell types derived from pluripotent stem cells (hiPSCs) recapitulate aspects of human physiology better than standard two-dimensional models in vitro. They typically consist of less than 5000 cells and are used to measure contraction kinetics although...journal article 2023
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Carrete, Israel A. (author), Ghodrat, S. (author), Scherer, Diana (author), Karana, E. (author)Plant root growth can be altered by introducing obstacles in the path of growth. This principle is used in design to produce planar grid structures composed of interweaving roots. The Engineered Plant Root Materials (EPRMs) grown with this method have the potential to serve as environmentally sensitive alternatives for conventional materials,...journal article 2023
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Bennis, N.X. (author), Anderson, Jonah P. (author), Kok, Siebe M.C. (author), Daran, J.G. (author)ErCas12a is a class 2 type V CRISPR-Cas nuclease isolated from Eubacterium rectale with attractive fundamental characteristics, such as RNA self-processing capability, and lacks reach-through royalties typical for Cas nucleases. This study aims to develop a ErCas12a-mediated genome editing tool applicable in the model yeast Saccharomyces...journal article 2023
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Hai, R. (author), Koutras, C. (author), Quix, Christoph (author), Jarke, Matthias (author)Data lakes are becoming increasingly prevalent for Big Data management and data analytics. In contrast to traditional 'schema-on-write' approaches such as data warehouses, data lakes are repositories storing raw data in its original formats and providing a common access interface. Despite the strong interest raised from both academia and...journal article 2023
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Ganapathy, S. (author), Meng, X. (author), Mossel, D.D.M. (author), Jagt, Mels (author), Brinks, D. (author)Genetically encoded voltage indicators, particularly those based on microbial rhodopsins, are gaining traction in neuroscience as fluorescent sensors for imaging voltage dynamics with high-spatiotemporal precision. Here we establish a novel genetically encoded voltage indicator candidate based on the recently discovered subfamily of the...journal article 2023
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Ribas, Francesca (author), Portos-Amill, Laura (author), Falqués, Albert (author), Arriaga, Jaime (author), Marcos, Marta (author), Ruessink, Gerben (author)Mean sea-level rise (MSLR) will induce shoreline recession, increasing the stress on coastal systems of high socio-economic and environmental values. Localized mega-nourishments are meant to alleviate erosion problems by diffusing alongshore over decades and thus feeding adjacent beaches. The 21-st century morphological evolution of the...journal article 2023
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Mitici, M.A. (author), de Pater, I.I. (author), Barros, Anne (author), Zeng, Zhiguo (author)The increasing availability of condition-monitoring data for components/systems has incentivized the development of data-driven Remaining Useful Life (RUL) prognostics in the past years. However, most studies focus on point RUL prognostics, with limited insights into the uncertainty associated with these estimates. This limits the...journal article 2023
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Akolawala, Q. (author), Keuning, F.E. (author), Rovituso, M. (author), van Burik, Wouter (author), van der Wal, E.M. (author), Versteeg, Henri H. (author), Rondon, Araci M.R. (author), Accardo, A. (author)Glioblastoma (GBM) is a devastating cancer of the brain with an extremely poor prognosis. While X-ray radiotherapy and chemotherapy remain the current standard, proton beam therapy is an appealing alternative as protons can damage cancer cells while sparing the surrounding healthy tissue. However, the effects of protons on in vitro GBM models...journal article 2023
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Zampetti, Fiorelli (author), Tamburri, Damian A. (author), Panichella, Sebastiano (author), Panichella, A. (author), Di Penta, Massimiliano (author), Canfora, Gerardo (author)Continuous Integration and Delivery (CI/CD) practices have shown several benefits for software development and operations, such as faster release cycles and early discovery of defects. For Cyber-Physical System (CPS) development, CI/CD can help achieving required goals, such as high dependability, yet it may be challenging to apply. This...journal article 2022
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de Pater, I.I. (author), Reijns, Arthur (author), Mitici, M.A. (author)The increasing availability of condition monitoring data for aircraft components has incentivized the development of Remaining Useful Life (RUL) prognostics in the past years. However, only few studies consider the integration of such prognostics into maintenance planning. In this paper we propose a dynamic, predictive maintenance scheduling...journal article 2022
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Pande, S. (author), Haeffner, Melissa (author), Blöschl, Günter (author), Alam, M.F. (author), Castro, Cyndi (author), Di Baldassarre, Giuliano (author), Frick-Trzebitzky, Fanny (author), Hogeboom, Rick (author), Kreibich, Heidi (author), Mukherjee, Jenia (author)In a recent editorial in the journal Nature Sustainability, the editors raised the concern that journal submissions on water studies appear too similar. The gist of the editorial: “too many publications and not enough ideas.” In this response, we contest this notion, and point to the numerous new ideas that result from taking a broader view...journal article 2022
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Reinders, K.J. (author), Giardina, Giorgia (author), Zurfluh, Florian (author), Ryser, Jurg (author), Hanssen, R.F. (author)In the planning stage of new infrastructure or when designing renovation of existing infrastructure, information about existing slope movements or settlements is essential to make informed design decisions. Interferometric<br/>Synthetic Aperture Radar (InSAR) techniques can be of value to identify these risks in an early stage of a project....journal article 2022
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Azcarate Aguerre, J.F. (author), Klein, T. (author), Konstantinou, T. (author), Veerman, Martijn (author)The servitisation of the built environment, through the implementation of product–service systems, is considered a promising business strategy to achieve a circular economy transition. This servitisation faces a number of practical challenges, among them the technological readiness and effective integration and application of existing and...journal article 2022
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Hilberath, T. (author), van Troost, A.G.L. (author), Alcalde, Miguel (author), Hollmann, F. (author)The use of water-miscible organic co-solvents in biocatalysis is a simple procedure for obtaining higher enzymatic activities toward hydrophobic substrates. However, effects on activity and stability have to be carefully evaluated, also with regard to the type and concentration of the respective co-solvent. In this contribution, we investigated...journal article 2022
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Fu, J. (author), Nunez, Alfredo (author), De Schutter, B.H.K. (author)Natural disasters pose a tremendous risk to the reliability of distribution networks. In this paper, a novel real-time UAV routing strategy for coordination between monitoring and inspection for post-disaster restoration in distribution networks is proposed. With our proposed real-time UAV routing strategy, damages can be inspected by UAVs...journal article 2022
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Kula, E. (author), Greuter, Eric (author), van Deursen, A. (author), Gousios, G. (author)Late delivery of software projects and cost overruns have been common problems in the software industry for decades. Both problems are manifestations of deficiencies in effort estimation during project planning. With software projects being complex socio-technical systems, a large pool of factors can affect effort estimation and on-time...journal article 2022
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Li, Guoqiang (author), Gorges, Daniel (author), Wang, M. (author)In this paper a learning-based optimization method for online gear shift and velocity control is presented to reduce the fuel consumption and improve the driving comfort in a car-following process. The continuous traction force and the discrete gear shift are optimized jointly to improve both the powertrain operation and the longitudinal...journal article 2022