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Nagalingam, Nagaraj (author), Raghunathan, Aswin (author), Korede, V.B. (author), Overmars, E.F.J. (author), Hung, S. (author), Hartkamp, Remco (author), Padding, J.T. (author), Smith, C.S. (author), Eral, H.B. (author)Optofluidic devices have revolutionized the manipulation and transportation of fluid at smaller length scales ranging from micrometers to millimeters. We describe a dedicated optical setup for studying laser-induced cavitation inside a microchannel. In a typical experiment, we use a tightly focused laser beam to locally evaporate the solution...journal article 2023
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Dutta, S. (author), Bieling, T.J. (author), Verbiest, G.J. (author)Fluid flow processes such as drainage and evaporation in porous media are crucial in geological and biological systems. The motion of the displacement front of a moving fluid through multi-phase interfaces is often associated with abrupt mechanical energy release, detectable as acoustic emissions (AEs). The exact origin of these pulses and...journal article 2023
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Neves Sao Pedro, M. (author), Isaksson, Madelène (author), Gomis-Fons, Joaquín (author), Eppink, Michel H.M. (author), Nilsson, Bernt (author), Ottens, M. (author)The implementation of continuous processing in the biopharmaceutical industry is hindered by the scarcity of process analytical technologies (PAT). To monitor and control a continuous process, PAT tools will be crucial to measure real-time product quality attributes such as protein aggregation. Miniaturizing these analytical techniques can...journal article 2023
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Kurniawan, Tetuko (author), Sahebdivani, Mahsa (author), Zaremba, Damian (author), Blonski, Slawomir (author), Garstecki, Piotr (author), van Steijn, V. (author), Korczyk, Piotr M. (author)Two decades of research on droplet formation in microchannels have led to the widely accepted view that droplets form through the squeezing mechanism when interfacial forces dominate over viscous forces. The initially surprising finding that the volume of the droplets is insensitive to the relative importance of these two forces is nowadays...journal article 2023
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Blankespoor, Maarten (author), Manzaneque Garcia, T. (author), Ghatkesar, M.K. (author)The capacity to precisely pipette femtoliter volumes of liquid enables many applications, for example, to functionalize a nanoscale surface and manipulate fluids inside a single-cell. A pressure-controlled pipetting method is the most preferred, since it enables the widest range of working liquids. However, precisely controlling femtoliter...journal article 2023
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Trapp, S. (author), Lammers, Tom (author), Engudar, Gokce (author), Hoehr, Cornelia (author), Denkova, A.G. (author), Paulssen, E. (author), de Kruijff, R.M. (author)Background: The radionuclide Ga-68 is commonly used in nuclear medicine, specifically in positron emission tomography (PET). Recently, the interest in producing Ga-68 by cyclotron irradiation of [<sup>68</sup>Zn]Zn nitrate liquid targets is increasing. However, current purification methods of Ga-68 from the target solution consist of multi...journal article 2023
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Application of a fluorescent dye-based microfluidic sensor for real-time detection of mAb aggregatesNeves Sao Pedro, M. (author), Eppink, Michel H.M. (author), Ottens, M. (author)The lack of process analytical technologies able to provide real-time information and process control over a biopharmaceutical process has long impaired the transition to continuous biomanufacturing. For the monoclonal antibody (mAb) production, aggregate formation is a major critical quality attribute (CQA) with several known process...journal article 2023
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Tsan, Vi hung (author), Fan, D. (author), Caneva, S. (author), Smith, C.S. (author), Verbiest, G.J. (author)A low-cost glass-based microfluidic flow cell with a piezo actuator is built using off-the-shelf parts (total cost €9 per device) to apply acoustophoretic force on polystyrene micro-beads. The main challenge in the fabrication of these devices was to ensure their leak tightness, which we solved using double-sided tape and nail polish. Beads...journal article 2023
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Aghaseyedi, Maryam (author), Salehi, Alireza (author), Valijam, Shayan (author), Shooshtari, M. (author)A microfluidic-based gas sensor was chosen as an alternative method to gas chromatography and mass spectroscopy systems because of its small size, high accuracy, low cost, etc. Generally, there are some parameters, such as microchannel geometry, that affect the gas response and selectivity of the microfluidic-based gas sensors. In this study, we...journal article 2022
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Campos de Oliveira, A. (author), Pan, Sining (author), Wiegerink, Remco J. (author), Makinwa, K.A.A. (author)This article presents a microelectromechanical system (MEMS) Coriolis-based mass-flow-to-digital converter (Φ DC) that can be used with both liquids and gases. It consists of a micromachined Coriolis mass flow sensor and a CMOS interface circuit that drives it into oscillation and digitizes the resulting mass flow information. A phase-locked...journal article 2022
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Büke, F. (author), Grilli, Jacopo (author), Cosentino Lagomarsino, Marco (author), Bokinsky, G.E. (author), Tans, S.J. (author)Growth and division are central to cell size. Bacteria achieve size homeostasis by dividing when growth has added a constant size since birth, termed the adder principle, by unknown mechanisms.<sup>1</sup><sup>,</sup><sup>2</sup> Growth is well known to be regulated by guanosine tetraphosphate (ppGpp), which controls diverse processes from...journal article 2022
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Zhang, Shuang (author), Tuk, Bastiaan (author), van de Peppel, Jeroen (author), Kremers, Gert Jan (author), Koedam, Marijke (author), Pesch, G.R. (author), Rahman, Z. (author), Hoogenboezem, Remco M. (author), Boukany, P. (author)A functional vascular system is a prerequisite for bone repair as disturbed angiogenesis often causes non-union. Paracrine factors released from human bone marrow derived mesenchymal stromal cells (BMSCs) have angiogenic effects on endothelial cells. However, whether these paracrine factors participate in blood flow dynamics within bone...journal article 2022
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van Rooijen, W.A. (author), Hashemi, L. (author), Boon, M.M. (author), Farajzadeh, R. (author), Hajibeygi, H. (author)Underground Hydrogen Storage (UHS) is an attractive technology for large-scale (TWh) renewable energy storage. To ensure the safety and efficiency of the UHS, it is crucial to quantify the H<sub>2</sub> interactions with the reservoir fluids and rocks across scales, including the micro scale. This paper reports the experimental measurements...journal article 2022
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van Putten, R. (author), Eyke, Natalie S. (author), Baumgartner, L.M. (author), Schultz, Victor L. (author), Filonenko, G.A. (author), Jensen, Klavs F. (author), Pidko, E.A. (author)Automation and microfluidic tools potentially enable efficient, fast, and focused reaction development of complex chemistries, while minimizing resource- and material consumption. The introduction of automation-assisted workflows will contribute to the more sustainable development and scale-up of new and improved catalytic technologies....journal article 2022
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Neves Sao Pedro, M. (author), Silva dos Santos, M. (author), Eppink, Michel H.M. (author), Ottens, M. (author)A major challenge in the transition to continuous biomanufacturing is the lack of process analytical technology (PAT) tools which are able to collect real-time information on the process and elicit a response to facilitate control. One of the critical quality attributes (CQAs) of interest during monoclonal antibodies production is aggregate...journal article 2022
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Picanço Castanheira Da Silva, T. (author), Eppink, Michel (author), Ottens, M. (author)Biopharmaceuticals are becoming increasingly important in modern healthcare. Monoclonal antibodies (mAb) are one of the most widely used therapeutic proteins and are important for the treatment of cancer and autoimmune diseases, among others. After cell culture there are still large amounts of other impurities (e.g. host cell proteins) in...journal article 2022
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Amador, G.J. (author), van Dijk, Dennis (author), Kieffer, R.Y. (author), Aubin-Tam, M.E. (author), Tam, D.S.W. (author)Vital biological processes, such as trafficking, sensing, and motility, are facilitated by cellular lipid membranes, which interact mechanically with surrounding fluids. Such lipid membranes are only a few nanometers thick and composed of a liquid crystalline structure known as the lipid bilayer. Here, we introduce an active, noncontact, two...journal article 2021
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Neves Sao Pedro, M. (author), Picanço Castanheira Da Silva, T. (author), Patil, Rohan (author), Ottens, M. (author)Continuous manufacturing is an indicator of a maturing industry, as can be seen by the example of the petrochemical industry. Patent expiry promotes a price competition between manufacturing companies, and more efficient and cheaper processes are needed to achieve lower production costs. Over the last decade, continuous biomanufacturing has...journal article 2021
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Van Delft, Falco C.M.J.M. (author), Sudalaiyadum Perumal, Ayyappasamy (author), van Langen-Suurling, A.K. (author), de Boer, C.R. (author), Kašpar, Ondřej (author), Tokárová, Viola (author), Dirne, F. (author), Nicolau, Dan V. (author)Non-deterministic polynomial (NP-) complete problems, whose number of possible solutions grows exponentially with the number of variables, require by necessity massively parallel computation. Because sequential computers, such as solid state-based ones, can solve only small instances of these problems within a reasonable time frame, parallel...journal article 2021
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Totlani, K. (author), Wang, Yen Chieh (author), Bisschops, Maxime (author), de Riese, Thorben (author), Kreutzer, M.T. (author), van Gulik, W.M. (author), van Steijn, V. (author)A key bottleneck in bioprocess development is that state-of-the-art tools used for screening of cells and optimization of cultivation conditions do not represent the conditions enforced at industrial scale. At industrial scale, cell growth is strictly controlled (“fed-batch”) to optimize the metabolites produced by the cells. In contrast,...journal article 2021