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Imani, Amin (author), Rahimi, E. (author), Lekka, Maria (author), Andreatta, Francesco (author), Magnan, Michele (author), Gonzalez Garcia, Y. (author), Mol, J.M.C. (author), Raman, R. K. Singh (author), Fedrizzi, Lorenzo (author), Asselin, Edouard (author)Mg and its alloys are promising biodegradable materials for orthopedic implants and cardiovascular stents. The first interactions of protein molecules with Mg alloy surfaces have a substantial impact on their biocompatibility and biodegradation. We investigate the early-stage electrochemical, chemical, morphological, and electrical surface...journal article 2024
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Chen, L.M. (author), Beck, Paula (author), van Ede, J.M. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Abstract: Anionic polymers, such as heparin, have been widely applied in the chemical and medical fields, particularly for binding proteins (e.g., fibroblast growth factor 2 (FGF-2) and histones). However, the current animal-based production of heparin brings great risks, including resource shortages and product contamination. Recently,...journal article 2024
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Yin, Xinjian (author), Gong, Wenzhong (author), Zeng, Yujing (author), Qiu, Hulin (author), Liu, Lan (author), Hollmann, F. (author), Chen, Bishuang (author)The biocatalytic oxidative deamination of β-amino alcohols holds significant practical potential in kinetic resolution and/or deracemization process to access (R)-β-amino alcohols. This study exemplifies a notable instance of acquisition and utilization of this valuable oxidative deamination activity. Initially, the mutation N261M (M0) was...journal article 2024
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Pankratov, Dmitrii (author), Hidalgo Martinez, Silvia (author), Karman, Cheryl (author), Gerzhik, Anastasia (author), Gomila, Gabriel (author), Trashin, Stanislav (author), Boschker, H.T.S. (author), Geelhoed, Jeanine S. (author), Meysman, F.J.R. (author)Cable bacteria are filamentous, multicellular microorganisms that display an exceptional form of biological electron transport across centimeter-scale distances. Currents are guided through a network of nickel-containing protein fibers within the cell envelope. Still, the mechanism of long-range conduction remains unresolved. Here, we...journal article 2024
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Neijenhuis, T. (author), Le Bussy, Olivier (author), Geldhof, Geoffroy (author), Klijn, M.E. (author), Ottens, M. (author)Protein-based biopharmaceuticals require high purity before final formulation to ensure product safety, making process development time consuming. Implementation of computational approaches at the initial stages of process development offers a significant reduction in development efforts. By preselecting process conditions, experimental...journal article 2024
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Ingram, A.A. (author), Oike, K. (author)Astonishing progress has been achieved in unlocking new-to-nature biocatalysis in the past decades. The progress in protein engineering enabled research to efficiently incorporate artificial structural elements into enzyme design. Recent trends include cofactor mimetics, artificial metalloenzymes and non-canonical amino acids. In this...journal article 2024
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Xu, Yanghui (author), Wang, Xintu (author), Ou, Q. (author), Zhou, Zhongbo (author), van der Hoek, J.P. (author), Liu, G. (author)Increasing wildfire frequency, a consequence of global climate change, releases incomplete combustion byproducts such as aquatic pyrogenic dissolved organic matter (DOM) and black carbon (DBC) into waters, posing a threat to water security. In August 2022, a series of severe wildfires occurred in Chongqing, China. Samples from seven locations...journal article 2024
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Quispe Haro, Juan José (author), Chen, Fei (author), Los, R. (author), Shi, Shuqi (author), Sun, Wenjun (author), Chen, Yong (author), Idema, T. (author), Wegner, Seraphine V. (author)The transition of bacteria from an individualistic to a biofilm lifestyle profoundly alters their biology. During biofilm development, the bacterial cell-cell adhesions are a major determinant of initial microcolonies, which serve as kernels for the subsequent microscopic and mesoscopic structure of the biofilm, and determine the resulting...journal article 2024
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Hebbar, Akshatha (author), Dey, P. (author), Vatti, Anoop Kishore (author)The ability of neat deep eutectic solvents (DESs) to influence protein structure and function has gained considerable interest due to the unstable nature of enzymes or therapeutic proteins, which are often exposed to thermal, chemical, or mechanical stresses when handled at an industrial scale. In this study, we simulated a model globular...journal article 2023
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Xu, J. (author), Fahmy-Garcia, Shorouk (author), Wesdorp, Marinus A. (author), Forte, Lucia (author), De Luca, Claudio (author), Filardo, Giuseppe (author), Labberté, Margot (author), Kok, Joeri (author), Nickel, Joachim (author), van Osch, G.J.V.M. (author)Despite promising clinical results in osteochondral defect repair, a recently developed bi-layered collagen/collagen-magnesium-hydroxyapatite scaffold has demonstrated less optimal subchondral bone repair. This study aimed to improve the bone repair potential of this scaffold by adsorbing bone morphogenetic protein 2 (BMP-2) and/or platelet...journal article 2023
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Deng, Z. (author), Ferreira, Ana Lucia Morgado (author), Spanjers, H. (author), van Lier, J.B. (author)Bovine serum albumin (BSA) and casein (CAS) were used in batch tests to compare the protein degradation in the presence and absence of carbohydrates and volatile fatty acids (VFAs). The modified Gompertz model was applied to estimate reaction rates. The results showed that deamination was the rate-limiting step, with a rate ranging between 2...journal article 2023
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Furukita Baptista, I.B. (author), Schifferstein, Hendrik N.J. (author)The market growth of plant-based alternatives to animal food products pushes agencies around the world to discuss specific regulations regarding their communication, terminology, and packaging design. We created and tested 18 packages of plant-based milk and plant-based chicken meat varying the “animalness” of terminology, container, image,...journal article 2023
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Rizki, Z. (author), Ravesloot, Richard (author), van Beckhoven, Ruud (author), Ottens, M. (author)The ultrafiltration/diafiltration (UF/DF) process is a crucial step in the canola protein isolation process from rapeseed meal. The process involves using a multi-stage membrane system to separate components of the mixture. As diafiltration dilutes the feed stream in the ultrafiltration system, a large amount of diafiltration water is...journal article 2023
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Siverino, Claudia (author), Fahmy-Garcia, Shorouk (author), Niklaus, Viktoria (author), Kops, Nicole (author), Dolcini, Laura (author), Misciagna, Massimiliano Maraglino (author), Ridwan, Yanto (author), Farrell, Eric (author), van Osch, G.J.V.M. (author), Nickel, Joachim (author)Bone Morphogenetic proteins (BMPs) like BMP2 and BMP7 have shown great potential in the treatment of severe bone defects. In recent in vitro studies, BMP9 revealed the highest osteogenic potential compared to other BMPs, possibly due to its unique signaling pathways that differs from other osteogenic BMPs. However, in vivo the bone forming...journal article 2023
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Deng, Z. (author), Muñoz Sierra, Julian (author), Ferreira, Ana Lucia Morgado (author), Cerqueda Garcia, D. (author), Spanjers, H. (author), van Lier, J.B. (author)Treating protein-rich wastewater using cost-effective and simple-structured single-stage reactors presents several challenges. In this study, we applied an anaerobic sequencing batch reactor (AnSBR) to treat protein-rich wastewater from a slaughterhouse. We focused on identifying the key factors influencing the removal of chemical oxygen...journal article 2023
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Rahimi, E. (author), Kim, Donghoon (author), Offoiach, Ruben (author), Sanchis-Gual, Roger (author), Chen, Xiang Zhong (author), Taheri, P. (author), Gonzalez Garcia, Y. (author), Mol, J.M.C. (author), Fedrizzi, Lorenzo (author), Pané, Salvador (author), Lekka, Maria (author)Functional oxide nanoparticles are extensively utilized in the last decades for biomedical purposes due to their unique functional properties. Nevertheless, their biodegradation mechanism by biological species, particularly by proteins at oxide/protein interfaces, still remains limited. Here, a systematic approaches is provided to investigate...journal article 2023
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Olavarria, Karel (author), Sousa, Diana Z. (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)Utilizing anaerobic metabolisms for the production of biotechnologically relevant products presents potential advantages, such as increased yields and reduced energy dissipation. However, lower energy dissipation may indicate that certain reactions are operating closer to their thermodynamic equilibrium. While stoichiometric analyses and...journal article 2023
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Application of a fluorescent dye-based microfluidic sensor for real-time detection of mAb aggregatesNeves Sao Pedro, M. (author), Eppink, Michel H.M. (author), Ottens, M. (author)The lack of process analytical technologies able to provide real-time information and process control over a biopharmaceutical process has long impaired the transition to continuous biomanufacturing. For the monoclonal antibody (mAb) production, aggregate formation is a major critical quality attribute (CQA) with several known process...journal article 2023
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Tomas Martinez, S. (author), Zwolsman, Erwin J. (author), Merlier, Franck (author), Pabst, Martin (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Polyphosphate accumulating organisms (PAOs) are responsible for enhanced biological phosphate removal (EBPR) from wastewater, where they grow embedded in a matrix of extracellular polymeric substances (EPS). EPSs comprise a mixture of biopolymers like polysaccharides or (glyco)proteins. Despite previous studies, little is known about the...journal article 2023
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Gomez de Santos, Patricia (author), González-Benjumea, Alejandro (author), Fernandez-Garcia, Angela (author), Aranda, Carmen (author), Wu, Y. (author), But, A. (author), Molina-Espeja, Patricia (author), Zhang, W. (author), Hollmann, F. (author)The hydroxylation of fatty acids is an appealing reaction in synthetic chemistry, although the lack of selective catalysts hampers its industrial implementation. In this study, we have engineered a highly regioselective fungal peroxygenase for the ω-1 hydroxylation of fatty acids with quenched stepwise over-oxidation. One single mutation near...journal article 2023