Anaerobic digestion (AD) has been commercially operated worldwide in full scale as a resource recovery technology underpinning a circular economy. However, problems such as a long start-up time, or system instability, have been reported in response to operational shocks. These issues are usually linked to the dynamics of the functional microbiota in AD. Exploring the microbiota-functionality nexus (MFN) could be pivotal to understand the reasons behind these difficulties, and hence improving AD performance. Here we present a systematic MFN study based on 138 samples taken from 20 well-profiled lab-scale AD reactors operated for up to two years. All the reactors were operated in the same lab within the same period of time using the same methodology to harvest physio-chemical and molecular data, including key monitoring parameters, qPCR, and 16S sequencing results. The results showed a core bacterial microbiota prevailing in all reactor types, including Bacillus, Clostridium, Bacteroides, Eubacterium, Cytophaga, Anaerophaga, and Syntrophomonas, while various methanogens dominated different communities due to different inocula origins, reactor temperatures, or salinity levels. This core bacterial microbiota well correlated with biogas production (Pearson correlation coefficient of 0.481, p < 0.0001). Such strong correlation was even comparable to that between the biogas production and the methanogenic 16S rRNA gene content (Pearson correlation coefficient of 0.481, p < 0.0001). The results indicated that AD performance only modestly correlated with microbial diversity, a key governing factor. AD microbiota was neither functionally redundant nor plastic, and a high variety in communities can exhibit a strong difference in reactor performance. Our study demonstrates the importance of a core bacterial microbiota in AD and supports inspiring considerations for design, bioaugmentation, and operational strategies of AD reactors in the future.

This study provides a comparison of the sludge characteristics along the height of an upflow anaerobic sludge blanket (UASB) reactor in terms of sludge morphology, activity and stability. The main aim of this study was to identify the best location (i.e. where sludge is of lowest stability and/or highest concentration) in the sludge bed for conveying the sludge from the low temperature UASB reactor to a digester. The sludge profile was investigated by collecting sludge samples along the different heights of the UASB-anaerobic membrane bioreactor treating municipal wastewater. Results showed that total solids and volatile solids concentrations decreased with height, and the highest chemical oxygen demand concentration was observed at the bottom of the reactor. Active biomass remained near inlet of the reactor; whereas, non-active biomass consisted of loose, suspended particles and flocculents moved towards the top. This was confirmed by the high specific COD consumption rate near the inlet and poor specific COD biodegradation in the remaining portions of the bioreactor. Apparently, the assumption of a completely mixed sludge bed behavior for the UASB reactor, being part of an AnMBR system, does not hold for this type of reactor systems even at low temperatures, which makes the location in sludge bed from where the sludge is to be conveyed to the digester of operational importance. Considering the observed sludge bed stratification, the sludge to be recirculated from the UASB reactor to the digester is recommended to be taken from 40 to 50% of the sludge bed height.

BACKGROUND: Membrane technology is used for the treatment of produced water enabling high permeate quality to meet discharge standards or potable and irrigation water quality standards. In this study, the effect of hybrid membrane-activated carbon processes on the performance of nanofiltration (NF) and reverse osmosis (RO) membrane systems treating mixed oil and gas field produced water was investigated. Different pre-treatment alternatives including combinations of microfiltration unit and powdered activated carbon (PAC) or granular activated carbon (GAC) systems were applied prior to nanofiltration and reverse osmosis processes. RESULTS: The activated carbon process increased chemical oxygen demand (COD) and conductivity removal efficiencies due to the organic matter and ion adsorption capacity of activated carbon. PAC addition gave the best removal efficiencies for COD, whereas highest conductivity removals were obtained in studies with GAC addition. CONCLUSION: Differences obtained in the performance of different pre-treatment alternatives based on targeted pollutants and membrane characteristics confirmed synergetic/antagonistic effects between pre- and final membrane treatment.

Submerged and external anaerobic dynamic membrane bioreactors (AnDMBRs) have been compared in terms of removal efficiency, filtration characteristics and microbial community structure. High COD removal efficiencies were obtained with both submerged and external AnDMBRs. To obtain an effective dynamic membrane (DM) layer enabling high quality permeate, longer time was required in the external AnDMBR configuration compared to the submerged one. A difference in microbial community structure was identified using pyrosequencing analyses between the submerged and external AnDMBRs. The number of archaeal types decreased in the bulk sludge of the external AnDMBR. External sludge recirculation might have had a negative effect on the archaeal community in the bulk sludge of the external AnDMBR. However, the sludge recirculation in the external AnDMBR configuration led to a filtration at lower total filtration resistance and TMP in comparison to the submerged one at the same gas sparging rate. Results showed that the submerged AnDMBR system can provide a shorter start-up period, slightly better permeate quality in terms of COD concentration, and higher biogas production in comparison to the external one in gas-lift mode.

In the last 40 years, anaerobic sludge bed reactor technology has evolved from localized laboratory-scale trials to worldwide successful implementations in a variety of industries. High-rate sludge bed reactors are characterized by a very small footprint and high applicable volumetric loading rates. Best performances are obtained when the sludge bed consists of highly active and well settleable granular sludge. Sludge granulation provides a rich microbial diversity, high biomass concentration, high solids retention time, good settling characteristics, reduction in both operation costs and reactor volume, and high tolerance to inhibitors and temperature changes. However, sludge granulation cannot be guaranteed on every type of industrial wastewater. Especially in the last two decades, various types of high-rate anaerobic reactor configurations have been developed that are less dependent on the presence of granular sludge, and many of them are currently successfully used for the treatment of various kinds of industrial wastewaters worldwide. This study discusses the evolution of anaerobic sludge bed technology for the treatment of industrial wastewaters in the last four decades, focusing on granular sludge bed systems.

A laboratory scale external anaerobic dynamic membrane bioreactor (AnDMBR) treating high strength wastewater (influent COD ≈ 20 g/L) was operated to assess the effect of biogas sparging velocity (GSV) and hydraulic retention time (HRT) on removal efficiency and dynamic membrane (DM) filtration characteristics. An increase in GSV resulted in a decrease in DM filtration resistance. DM or cake layer was identified as the main contributor to the total filtration resistance. The external AnDMBR achieved over 99% COD removal efficiency irrespective of the GSV. The results showed that the DM formation process proceeded until a stable cake layer was reached. Reducing of HRT resulted in an increase in protein/carbohydrate ratio in soluble microbial products (SMP) and an increase in biomass concentration in the bioreactor. Therefore, HRT affected TMP and total filtration resistance in the AnDMBR. A high permeate quality was obtained by an effective DM layer at organic loading rates (OLRs) between 2 and 3.6 kg COD/m³ d. Based on the fluxes observed in this research, the filter cloth costs would be in the range of 0.17 €/m³ of treated wastewater. The investment and operational costs of the AnDMBRs are expected to be substantially lower than that of conventional membrane filtration.