In this paper, we introduce a novel passive physical anti-tampering Physical Unclonable Function (PUF) based on glitters that can protect an entire Integrated Circuit (IC) and/or Printable Circuit Board (PCB). A prototype of the proposed glitter based PUF has been developed. The glitters are dropped randomly in a resin layer during its formation and their positioning is used as the basis of a PUF. The PUF response is created by taking a picture inside the coating layer. To get a stable response resilient against noise and different temperature cycles, the picture is processed using filtering, image processing, and error correction. Using actual drill measurements, our findings indicate that even drilling with a 0.1mm diameter drill can be detected and lead to a wrong PUF response.