More electric aircraft (MEA) is an important direction for future aircraft development. The printed circuit boards (PCBs) of power electronics equipment in MEA operate in a complex environment of low air pressure, square wave voltage, compact layout, and strong electromagnetic interference, which makes the PCB more prone to partial discharge (PD). However, the effective detection method and related discharge characteristics for PCB PD under square wave voltage are still unclear. Therefore, this article proposes a PCB PD detection method based on fluorescent optical fiber, which has good anti-electromagnetic interference ability. The PD characteristics of the PCB with three typical structures under three air pressures are studied, including PD nonbreakdown characteristics, changes in optical pulse amplitude and pulse repetition rate during PD aging, PD breakdown path analysis, and PCB surface electric field simulation. The analysis shows that air pressure, space charge, and PCB surface flatness all have important influences on the PD of PCB under square wave voltage. It provides important theoretical support for the insulation protection design and fault diagnosis of PCB for MEA in the future.