Nonlinear sound-sheet microscopy

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Nonlinear sound-sheet microscopy

Imaging opaque organs at the capillary and cellular scale

Journal Article (2025)

Author(s)

B.G. Heiles (TU Delft - ImPhys/Maresca group)

Flora Nelissen (Netherlands Institute for Neuroscience)

Rick Waasdorp (TU Delft - ImPhys/Maresca group)

Dion Terwiel (TU Delft - ImPhys/Maresca group)

Byung Min Park (TU Delft - ImPhys/Maresca group)

E. Muñoz-Ibarra (TU Delft - ImPhys/Maresca group)

A. Matalliotakis (TU Delft - ImPhys/Verweij group)

T. Ara (TU Delft - ImPhys/Medical Imaging)

Pierina Barturen-Larrea (California Institute of Technology)

Mengtong Duan (California Institute of Technology)

Mikhail G. Shapiro (Howard Hughes Medical Institute, California Institute of Technology)

Valeria Gazzola (Netherlands Institute for Neuroscience, Universiteit van Amsterdam)

D. Maresca (TU Delft - ImPhys/Maresca group, TU Delft - ImPhys/Medical Imaging)

Research Group

ImPhys/Maresca group

DOI related publication

https://doi.org/10.1126/science.ads1325

To reference this document use:

https://resolver.tudelft.nl/uuid:26a0da11-edb7-4f95-a902-fccbd5467739

More Info

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Publication Year

2025

Language

English

Research Group

ImPhys/Maresca group

Bibliographical Note

Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.@en

Issue number

6742

Volume number

388

Reuse Rights

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Abstract

Light-sheet fluorescence microscopy has revolutionized biology by visualizing dynamic cellular processes in three dimensions. However, light scattering in thick tissue and photobleaching of fluorescent reporters limit this method to studying thin or translucent specimens. In this study, we applied nondiffractive ultrasound beams in conjunction with a cross-amplitude modulation sequence and nonlinear acoustic reporters to enable fast and volumetric imaging of targeted biological functions. We reported volumetric imaging of tumor gene expression at the cubic centimeter scale using genetically encoded gas vesicles and localization microscopy of cerebral capillary networks using intravascular microbubble contrast agents. Nonlinear sound-sheet microscopy provides a ~64× acceleration in imaging speed, ~35× increase in imaged volume, and ~4× increase in classical imaging resolution compared with the state of the art in biomolecular ultrasound.

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File under embargo until 04-10-2025