EM
E. Muñoz-Ibarra
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2 records found
1
Journal article
(2026)
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R. Waasdorp, E. Muñoz-Ibarra, F. Nelissen, B. Heiles, V. Gazzola, D. Maresca, G. Renaud
The development of fully noninvasive, transcranial functional ultrasound (fUS) would increase the translatability and clinical potential of this neuroimaging modality. Unfortunately, transcranial fUS is hindered by skull-induced aberrations which degrade the power Doppler image quality and lower sensitivity. As a result, a majority of fUS imaging studies rely on craniotomies or acoustically transparent cranial windows. To advance fUS technology further, we present an adaptive aberration correction method based on ray-tracing through four tissue layers (transducer lens, gel and skin, bone, and brain tissue). Our method segments these layers and estimates ultrasound wave speeds in each layer iteratively. Once a velocity model of the imaging plane of interest is retrieved, ultrafast power Doppler imaging of the brain is performed using a ray-tracing beamformer that accounts for wave refraction. We tested our method in three adult rats, and estimated wave speeds for the skin/gel layer ( 1628±7 m/s ), skull bone ( 3247±110 m/s ), and brain tissue ( 1526±55 m/s ). After aberration correction, we measured an average adult rat skull thickness of 388±41μ m in agreement with anatomical records. The largest improvements in the Doppler imaging quality were observed in cortical brain layers adjacent to the skull; specifically, lateral spatial resolution was improved by 32 %. Our method consistently outperformed Doppler imaging based on traditional delay-and-sum (DAS) beamforming, which assumes a uniform sound speed.
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The development of fully noninvasive, transcranial functional ultrasound (fUS) would increase the translatability and clinical potential of this neuroimaging modality. Unfortunately, transcranial fUS is hindered by skull-induced aberrations which degrade the power Doppler image quality and lower sensitivity. As a result, a majority of fUS imaging studies rely on craniotomies or acoustically transparent cranial windows. To advance fUS technology further, we present an adaptive aberration correction method based on ray-tracing through four tissue layers (transducer lens, gel and skin, bone, and brain tissue). Our method segments these layers and estimates ultrasound wave speeds in each layer iteratively. Once a velocity model of the imaging plane of interest is retrieved, ultrafast power Doppler imaging of the brain is performed using a ray-tracing beamformer that accounts for wave refraction. We tested our method in three adult rats, and estimated wave speeds for the skin/gel layer ( 1628±7 m/s ), skull bone ( 3247±110 m/s ), and brain tissue ( 1526±55 m/s ). After aberration correction, we measured an average adult rat skull thickness of 388±41μ m in agreement with anatomical records. The largest improvements in the Doppler imaging quality were observed in cortical brain layers adjacent to the skull; specifically, lateral spatial resolution was improved by 32 %. Our method consistently outperformed Doppler imaging based on traditional delay-and-sum (DAS) beamforming, which assumes a uniform sound speed.
Nonlinear sound-sheet microscopy
Imaging opaque organs at the capillary and cellular scale
Journal article
(2025)
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Baptiste Heiles, Flora Nelissen, Mikhail G. Shapiro, Valeria Gazzola, David Maresca, Rick Waasdorp, Dion Terwiel, Byung Min Park, Eleonora Munoz Ibarra, Agisilaos Matalliotakis, Tarannum Ara, Pierina Barturen-Larrea, Mengtong Duan
Light-sheet fluorescence microscopy has revolutionized biology by visualizing dynamic cellular processes in three dimensions. However, light scattering in thick tissue and photobleaching of fluorescent reporters limit this method to studying thin or translucent specimens. In this study, we applied nondiffractive ultrasound beams in conjunction with a cross-amplitude modulation sequence and nonlinear acoustic reporters to enable fast and volumetric imaging of targeted biological functions. We reported volumetric imaging of tumor gene expression at the cubic centimeter scale using genetically encoded gas vesicles and localization microscopy of cerebral capillary networks using intravascular microbubble contrast agents. Nonlinear sound-sheet microscopy provides a ~64× acceleration in imaging speed, ~35× increase in imaged volume, and ~4× increase in classical imaging resolution compared with the state of the art in biomolecular ultrasound.
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Light-sheet fluorescence microscopy has revolutionized biology by visualizing dynamic cellular processes in three dimensions. However, light scattering in thick tissue and photobleaching of fluorescent reporters limit this method to studying thin or translucent specimens. In this study, we applied nondiffractive ultrasound beams in conjunction with a cross-amplitude modulation sequence and nonlinear acoustic reporters to enable fast and volumetric imaging of targeted biological functions. We reported volumetric imaging of tumor gene expression at the cubic centimeter scale using genetically encoded gas vesicles and localization microscopy of cerebral capillary networks using intravascular microbubble contrast agents. Nonlinear sound-sheet microscopy provides a ~64× acceleration in imaging speed, ~35× increase in imaged volume, and ~4× increase in classical imaging resolution compared with the state of the art in biomolecular ultrasound.