The emerging landscape of single-molecule protein sequencing technologies
Peggy Bohländer (TU Delft - ChemE/Advanced Soft Matter)
Mike Filius (Kavli institute of nanoscience Delft, TU Delft - BN/Chirlmin Joo Lab)
Xander F. van Kooten (Technion)
Adam Pomorski (Kavli institute of nanoscience Delft, TU Delft - BN/Chirlmin Joo Lab)
Sonja Schmid (Wageningen University & Research)
Cees Dekker (TU Delft - BN/Cees Dekker Lab, Kavli institute of nanoscience Delft)
Rienk Eelkema (TU Delft - ChemE/Advanced Soft Matter)
Sung Hyun Kim (Kavli institute of nanoscience Delft, TU Delft - BN/Chirlmin Joo Lab)
Chirlmin Joo (Kavli institute of nanoscience Delft, TU Delft - BN/Chirlmin Joo Lab)
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Abstract
Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.