Photochemical regeneration of flavoenzymes – An Old Yellow Enzyme case-study
M. C.R. Rauch (TU Delft - BT/Biocatalysis)
Mieke M.E. Huijbers (TU Delft - BT/Biocatalysis)
Martin Pabst (OLD BT/Cell Systems Engineering)
Caroline E. Paul (TU Delft - BT/Biocatalysis)
M. Pešić (TU Delft - BT/Biocatalysis)
I.W.C.E. Arends (Universiteit Utrecht, TU Delft - BT/Biocatalysis)
F. Hollmann (TU Delft - BT/Biocatalysis)
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Abstract
Direct, NAD(P)H-independent regeneration of Old Yellow Enzymes represents an interesting approach for simplified reaction schemes for the stereoselective reduction of conjugated C=C-double bonds. Simply by illuminating the reaction mixtures with blue light in the presence of sacrificial electron donors enables to circumvent the costly and unstable nicotinamide cofactors and a corresponding regeneration system. In the present study, we characterise the parameters determining the efficiency of this approach and outline the current limitations. Particularly, the photolability of the flavin photocatalyst and the (flavin-containing) biocatalyst represent the major limitation en route to preparative application.
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