Print Email Facebook Twitter Single image Fourier ring correlation Title Single image Fourier ring correlation Author Rieger, B. (TU Delft ImPhys/Computational Imaging; TU Delft ImPhys/Rieger group) Droste, I.E.A.C. (TU Delft ImPhys/Rieger group) Gerritsma, F.A. (TU Delft QN/vanderSarlab) Brink, Tip Ten (Student TU Delft) Stallinga, S. (TU Delft ImPhys/Stallinga group) Date 2024 Abstract We address resolution assessment for (light super-resolution) microscopy imaging. In modalities where imaging is not diffraction limited, correlation between two noise independent images is the standard way to infer the resolution. Here we take away the need for two noise independent images by computationally splitting one image acquisition into two noise independent realizations. This procedure generates two Poisson noise distributed images if the input is Poissonian distributed. As most modern cameras are shot-noise limited this procedure is directly applicable. However, also in the presence of readout noise we can compute the resolution faithfully via a correction factor. We evaluate our method on simulations and experimental data of widefield microscopy, STED microscopy, rescan confocal microscopy, image scanning microscopy, conventional confocal microscopy, and transmission electron microscopy. In all situations we find that using one image instead of two results in the same computed image resolution. To reference this document use: http://resolver.tudelft.nl/uuid:0a7f8f30-8a9a-4cf3-8a4d-383557433872 DOI https://doi.org/10.1364/OE.524683 ISSN 1094-4087 Source Optics Express, 32 (12), 21767-21782 Part of collection Institutional Repository Document type journal article Rights © 2024 B. Rieger, I.E.A.C. Droste, F.A. Gerritsma, Tip Ten Brink, S. Stallinga Files PDF oe-32-12-21767.pdf 7.12 MB Close viewer /islandora/object/uuid:0a7f8f30-8a9a-4cf3-8a4d-383557433872/datastream/OBJ/view