Design of a miniature gas exchanger for oxygen control in microfluids

Design of a miniature gas exchanger for oxygen control in microfluids: Design, integration, fabrication and validation of a miniature cell medium gas exchanger to control (sub)physiological oxygen concentrations on a liver chip

van Reeuwijk, Matthijs (TU Delft Mechanical, Maritime and Materials Engineering; TU Delft Micro and Nano Engineering)

Staufer, U. (mentor)
Langelaar, M. (mentor)
Raat, N.J.H. (mentor)

Delft University of Technology

Mechanical Engineering | Micro and Nano Engineering

2021-07-22

Current technology of Organ-on-Chips allows investigation of human cells outside the body including a microfluidic medium flow. Control of oxygen in this microfluidic flow is required to mimic the situation as in the human liver (physiological) or to create temporary low oxygen concentrations (hypoxia). In this research, a gas exchanger module is designed for liver-on-a-chip cell experiments at the Erasmus MC. Based on prototype tests and COMSOL simulations, the gas exchanger module is designed and integrated with a commercial Micronit microfluidic channel. The functioning of the fabricated gas exchanger is validated by measuring an oxygen gradient as well as performing oxygen consumption measurements on a liver cell layer.

oxygen control
liver-on-a-chip
hepatocytes
oxygen gradient
gas exchange
microfluidics
Organ-on-Chip

http://resolver.tudelft.nl/uuid:f13f7d0d-7179-4908-9f26-cf317a95f281

Student theses

master thesis

© 2021 Matthijs van Reeuwijk