Print Email Facebook Twitter Computational imaging modalities for multi-focal whole-slide imaging systems Title Computational imaging modalities for multi-focal whole-slide imaging systems Author van der Graaff, L. (TU Delft ImPhys/Computational Imaging) Van Leenders, Geert J.L.H. (Erasmus MC) Boyaval, Fanny (Leiden University Medical Center) Stallinga, S. (TU Delft ImPhys/Imaging Physics) Department ImPhys/Imaging Physics Date 2020 Abstract Whole-slide imaging systems can generate full-color image data of tissue slides efficiently, which are needed for digital pathology applications. This paper focuses on a scanner architecture that is based on a multi-line image sensor that is tilted with respect to the optical axis, such that every line of the sensor scans the tissue slide at a different focus level. This scanner platform is designed for imaging with continuous autofocus and inherent color registration at a throughput of the order of 400 MPx/s. Here, single-scan multi-focal whole-slide imaging, enabled by this platform, is explored. In particular, two computational imaging modalities based on multi-focal image data are studied. First, 3D imaging of thick absorption stained slides (∼60 μm) is demonstrated in combination with deconvolution to ameliorate the inherently weak contrast in thick-Tissue imaging. Second, quantitative phase tomography is demonstrated on unstained tissue slides and on fluorescently stained slides, revealing morphological features com-plementary to features made visible with conventional absorption or fluorescence stains. For both computational approaches simplified algorithms are proposed, targeted for straightforward parallel processing implementation at ∼GPx=s throughputs. To reference this document use: http://resolver.tudelft.nl/uuid:f32bd770-3f96-4332-83cf-ee8b00c8045e DOI https://doi.org/10.1364/AO.394290 Embargo date 2021-01-06 ISSN 1559-128X Source Applied Optics, 59 (20), 5967-5982 Bibliographical note Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public. Part of collection Institutional Repository Document type journal article Rights © 2020 L. van der Graaff, Geert J.L.H. Van Leenders, Fanny Boyaval, S. Stallinga Files PDF ao_59_20_5967taverne.pdf 4.42 MB Close viewer /islandora/object/uuid:f32bd770-3f96-4332-83cf-ee8b00c8045e/datastream/OBJ/view