- document
-
Moosabeiki, Vahid (author), Yarali, E. (author), Ghalayaniesfahani, A. (author), Callens, S.J.P. (author), van Manen, T. (author), Accardo, A. (author), Ghodrat, S. (author), Bico, José (author), Habibi, Mehdi (author), Mirzaali, Mohammad J. (author), Zadpoor, A.A. (author)Emerging 4D printing techniques have enabled the realization of smart materials whose shape or properties can change with time. Two important phenomena play important roles in the 4D printing of shape memory polymeric materials. First, the anisotropic deformation of the printed filaments due to residual stresses can be harnessed to create out...journal article 2024
- document
-
Li, Jiayu (author), Accardo, A. (author), Liu, Shutian (author)Micro/nanoscale additive manufacturing provides a powerful tool for advanced materials and structures with complex and precise features. For instance, the feature resolution of two-photon polymerization (2PP) can reach 200 nm. At this scale, materials properties can change, and the influence of the size effect cannot be ignored. Therefore, it...journal article 2024
- document
-
Yarali, E. (author), Klimopoulou, M. (author), David, M.K. (author), Boukany, P. (author), Staufer, U. (author), Fratila-Apachitei, E.L. (author), Zadpoor, A.A. (author), Accardo, A. (author), Mirzaali, Mohammad J. (author)The Poisson's ratio and elastic modulus are two parameters determining the elastic behavior of biomaterials. While the effects of elastic modulus on the cell response is widely studied, very little is known regarding the effects of the Poisson's ratio. The micro-architecture of meta-biomaterials determines not only the Poisson's ratio but...journal article 2024
- document
-
van Altena, P.F.J. (author), Accardo, A. (author)The mechanical properties of two-photon-polymerised (2PP) polymers are highly dependent on the employed printing parameters. In particular, the mechanical features of elastomeric polymers, such as IP-PDMS, are important for cell culture studies as they can influence cell mechanobiological responses. Herein, we employed optical-interferometer...journal article 2023
- document
-
Sharaf, A.M.S.E. (author), Timmerman, Raissa (author), Bajramovic, Jeffrey (author), Accardo, A. (author)The most widely employed approach by cell biologists to performing in vitro cell culture assays is the one using 2D plastic culture ware systems, which allows reproducibility and ease of use. Moreover, this method is cost-effective. However, in most cases, these flat surfaces lead to the formation of unrealistic 2D cell monolayers, which do not...journal article 2023
- document
-
Sharaf, A.M.S.E. (author), Frimat, J. P. (author), Kremers, G. J. (author), Accardo, A. (author)Two-photon polymerization (2PP) has provided the field of cell biology with the opportunity to fabricate precisely designed microscaffolds for a wide range of studies, from mechanobiology to in vitro disease modelling. However, a multitude of commercial and in-house developed photosensitive materials employed in 2PP suffers from high auto...journal article 2023
- document
-
Yarali, E. (author), Zadpoor, A.A. (author), Staufer, U. (author), Accardo, A. (author), Mirzaali, Mohammad J. (author)Mechanical and morphological design parameters, such as stiffness or porosity, play important roles in creating orthopedic implants and bone substitutes. However, we have only a limited understanding of how the microarchitecture of porous scaffolds contributes to bone regeneration. Meta-biomaterials are increasingly used to precisely engineer...review 2023
- document
-
Akolawala, Q. (author), Keuning, F.E. (author), Rovituso, M. (author), van Burik, Wouter (author), van der Wal, E.M. (author), Versteeg, Henri H. (author), Rondon, Araci M.R. (author), Accardo, A. (author)Glioblastoma (GBM) is a devastating cancer of the brain with an extremely poor prognosis. While X-ray radiotherapy and chemotherapy remain the current standard, proton beam therapy is an appealing alternative as protons can damage cancer cells while sparing the surrounding healthy tissue. However, the effects of protons on in vitro GBM models...journal article 2023
- document
-
Sharaf, A.M.S.E. (author), Roos, B. (author), Timmerman, Raissa (author), Kremers, Gert-Jan (author), Bajramovic, Jeffrey John (author), Accardo, A. (author)Microglia are the resident macrophages of the central nervous system and contribute to maintaining brain’s homeostasis. Current 2D “petri-dish” in vitro cell culturing platforms employed for microglia, are unrepresentative of the softness or topography of native brain tissue. This often contributes to changes in microglial morphology, exhibiting...journal article 2022
- document
-
Barin, N. (author), Balcioglu, Hayri E. (author), de Heer, Iris (author), de Wit, Maurice (author), Lamfers, Martine L.M. (author), van Royen, Martin E. (author), French, Pim J. (author), Accardo, A. (author)A major obstacle in glioma research is the lack of in vitro models that can retain cellular features of glioma cells in vivo. To overcome this limitation, a 3D-engineered scaffold, fabricated by two-photon polymerization, is developed as a cell culture model system to study patient-derived glioma cells. Scanning electron microscopy, (live...journal article 2022
- document
-
Akolawala, Q. (author), Rovituso, M. (author), Versteeg, Henri H. (author), Rondon, Araci M.R. (author), Accardo, A. (author)Glioblastoma (GBM) is a devastating cancer of the brain with an extremely poor prognosis. For this reason, besides clinical and preclinical studies, novel in vitro models for the assessment of cancer response to drugs and radiation are being developed. In such context, three-dimensional (3D)-engineered cellular microenvironments, compared to...journal article 2022
- document
-
Neves Leal Costa, B. (author), Adão, Ricardo M.R. (author), Maibohm, Christian (author), Accardo, A. (author), Cardoso, Vanessa F. (author), Nieder, Jana B. (author)Biomimicking biological niches of healthy tissues or tumors can be achieved by means of artificial microenvironments, where structural and mechanical properties are crucial parameters to promote tissue formation and recreate natural conditions. In this work, three-dimensional (3D) scaffolds based on woodpile structures were fabricated by two...journal article 2022
- document
-
Aghajani, S. (author), Accardo, A. (author), Tichem, M. (author)One of the essential requirements to create nanoparticle (NP)-based applications and functions is the ability to control their deposition in specific locations. Many methods have been proposed, with wet direct writing (DW) techniques such as inkjet printing being the most employed. These methods generally depend on off-line and solvent-based...journal article 2022
- document
-
Castillo Ransanz, Lucía (author), van Altena, P.F.J. (author), Heine, Vivi M. (author), Accardo, A. (author)The biomechanical properties of the brain microenvironment, which is composed of different neural cell types, the extracellular matrix, and blood vessels, are critical for normal brain development and neural functioning. Stiffness, viscoelasticity and spatial organization of brain tissue modulate proliferation, migration, differentiation, and...review 2022
- document
-
Mirzaali, Mohammad J. (author), Ghorbani, Aref (author), Nakatani, Kenichi (author), Nouri Goushki, M. (author), Tümer, N. (author), Callens, S.J.P. (author), Janbaz, S. (author), Accardo, A. (author), Bico, José (author), Habibi, Mehdi (author), Zadpoor, A.A. (author)The design of advanced functional devices often requires the use of intrinsically curved geometries that belong to the realm of non-Euclidean geometry and remain a challenge for traditional engineering approaches. Here, it is shown how the simple deflection of thick meta-plates based on hexagonal cellular mesostructures can be used to achieve...journal article 2021
- document
-
Aghajani, S. (author), Accardo, A. (author), Tichem, M. (author)The ability to tune the localised surface plasmon resonance (LSPR) behaviour of metal nanostructures has great importance for many optical sensor applications such as metal (plasmon) enhanced fluorescence spectroscopy and surface-enhanced Raman scattering (SERS). In this paper, we used Aerosol Direct Writing (ADW) to selectively deposit fine...journal article 2021
- document
-
Babi, Mouhanad (author), Riesco, Roberto (author), Boyer, Louisa (author), Fatona, Ayodele (author), Accardo, A. (author), Malaquin, Laurent (author), Moran-Mirabal, Jose (author)In nature, cells exist in three-dimensional (3D) microenvironments with topography, stiffness, surface chemistry, and biological factors that strongly dictate their phenotype and behavior. The cellular microenvironment is an organized structure or scaffold that, together with the cells that live within it, make up living tissue. To mimic...journal article 2021
- document
-
Aghajani, S. (author), Accardo, A. (author), Tichem, M. (author)Surface-enhanced Raman scattering (SERS) substrates are of great interest for detecting low-concentrated analytes. However, issues such as multistep processing, cost, and possible presence of hazardous substances in the fabrication still represent a significant drawback. In this paper, an innovative direct writing method is introduced for...journal article 2020
- document
-
Fan, D. (author), Staufer, U. (author), Accardo, A. (author)The realization of biomimetic microenvironments for cell biology applications such as organ-on-chip, in vitro drug screening, and tissue engineering is one of the most fascinating research areas in the field of bioengineering. The continuous evolution of additive manufacturing techniques provides the tools to engineer these architectures at...journal article 2019