Metabolic engineering of Saccharomyces cerevisiae for C4-dicarboxylic acid production

Biotechnological production of chemicals from renewable feedstocks offers a sustainable alternative to petrochemistry. Understanding of the biology of microorganisms and plants is increasing at an unprecedented rate and tools with which these organisms can be engineered for industrial application are becoming ever more powerful. However,...

Dynamic response of Saccharomyces cerevisiae to fermentative growth conditions

Application and assessment of experimental methods to study the cytosolic primary metabolism of yeast

Although a large part of the metabolism of the yeast Saccharomyces cerevisiae, has been clarified, there are a number of issues that still limit the predictive power of metabolic models for this organism. The dissertation aims to improve the collection of data for metabolic models through application and assessment of different experimental...

Physiological impact and context dependency of transcriptional responses: A chemostat study in Saccharomyces cerevisiae

This thesis is a compilation of a four-year PhD project on bakers' yeast (Saccharomyces cerevisiae). Since the entire S. cerevisiae genome sequence became available in 1996, DNA-microarray analysis has become a popular high-information-density tool for analyzing gene expression in this important industrial microorganism and model eukaryote. This...

Robust experimental methods to study in-vivo pre-steady state kinetics of primary metabolism in Saccharomyces cerevisiae

In this thesis, robust experimental methods and experimental designs for quantitative analysis of the metabolome (concentrations of relevant metabolites in the cell) of Saccharomyces cerevisiae (Bakers yeast) have been presented. In this research, it is essential to obtain a snapshot of intracellular as well as extracellular metabolite...