Process for the recovery of lipids or hydrocarbons

The invention is directed to a method for recovering a lipid or hydrocarbon from a fermentation mixture, comprising the steps of - providing a fermentation mixture wherein the lipid or hydrocarbon is produced by microbial fermentation in a fermentation vessel, which mixture comprises an aqueous phase and a liquid product phase, wherein the...

Fermentation broth components influence droplet coalescence and hinder advanced biofuel recovery during fermentation

Developments in synthetic biology enabled the microbial production of long chain hydrocarbons, which can be used as advanced biofuels in aviation or transportation. Currently, these fuels are not economically competitive due to their production costs. The current process offers room for improvement: by utilizing lignocellulosic feedstock,...

Feasibility study of an alkaline-based chemical treatment for the purification of polyhydroxybutyrate produced by a mixed enriched culture

This study focused on investigating the feasibility of purifying polyhydroxybutyrate (PHB) from mixed culture biomass by alkaline-based chemical treatment. The PHB-containing biomass was enriched on acetate under non-sterile conditions. Alkaline treatment (0.2 M NaOH) together with surfactant SDS (0.2 w/v% SDS) could reach 99% purity, with more...

Theoretical performance of countercurrent reactors for production of enantiopure compounds

Irreversible reactions are being applied in enzymatic kinetic resolution to obtain enantiomerically pure compounds from racemic mixtures. Using model calculations for situations without mass transfer limitation, we show that reversible reactions might also be useful for enzymatic kinetic resolution, provided that countercurrent systems are used...

Process for the continuous biological production of lipids, hydrocarbons or mixtures thereof

The present invention is directed to a process for the continuous biological production of lipids, hydrocarbons, hydrocarbon like material or mixtures thereof by conversion of a suitable substrate using micro-organisms, in which process the said substrate is continuously, anaerobically fermented to produce lipids, hydrocarbons, hydrocarbon like...

Fumaric acid production by fermentation

Abstract The potential of fumaric acid as a raw material in the polymer industry and the increment of cost of petroleum-based fumaric acid raises interest in fermentation processes for production of this compound from renewable resources. Although the chemical process yields 112% w/w fumaric acid from maleic anhydride and the fermentation...

Phase Behavior of an Intact Monoclonal Antibody

Understanding protein phase behavior is important for purification, storage, and stable formulation of protein drugs in the biopharmaceutical industry. Glycoproteins, such as monoclonal antibodies (MAbs) are the most abundant biopharmaceuticals and probably the most difficult to crystallize among water-soluble proteins. This study explores the...

Method of preparing a protein aggregate and a pharmaceutical composition

The invention relates to a method of preparing a protein aggregate, wherein an aqueous protein solution is acidified with a pH that lies above the isoelectric point of the protein. In accordance with the invention, a first protein, which through acidificaiton is able to form a protein aggregate, is acidified in the presence of a second protein...

Method for the production of a fermentation product from an organism

The invention relates to a method for the production of a fermentation product from an organism cultured in a culture medium, wherein the organism after the formation of the fermentation product is subjected to a lysis treatment in the presence of a lysis-promoting compound. In accordance with the invention, the lysis-promoting agent is a...

Technical and Economical Feasibility of Production of Ethanol from Sugar Cane and Sugar Cane Bagasse

The primary aim of this study is to investigate and analyze the sugar-ethanol plants operating in Brazil to construct a raw model to gain better understanding and insight about the technical and economical aspects of the currently operating plants. And, the secondary aim is to combine the knowledge gathered from the currently existing plant with...

Method of preparing protein agglomerates, protein agglomerates, and a food product and a pharmaceutical composition in which they are comprised

The invention relates to a method for the preparation of protein agglomerates, by introducing CO2 in an aqueous protein-containing solution. According to the invention CO2 is gradually and while mixing supplied yielding spherical protein agglomerates, after which the pressure is reduced with a rate such that the spherical nature of the protein...

Method of substantially continuously separating two compounds using a moving bed or a simulated moving bed

The present invention relates to a method of separating a first solute A and a second solute B using (simulated) moving bed chromatography. According to the present invention at least one of a) a feedstream; and b) a desorbent stream comprises an organic solvent. The use of different solvent liquids for the feedstream and the desorbent stream...

A countercurrent adsorptive fluidized bed reactor for heterogeneous bioconversions

Method of carrying out a treatment in the presence of a centrifugal force and an apparatus therefor

Abstract of WO 9733687 (A1) The invention relates to a method and an apparatus for carrying out a treatment in the presence of centrifugal force. The treatment may be a physical or a chemical treatment. For this purpose the first and the second phase are brought into contact with each other. The second phase comprises a phase soluble in the...