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Breve, T. (author), Liu, H. (author), Denkova, A.G. (author), Eelkema, R. (author)Gamma radiation triggered secondary crosslinking of dextran hydrogels leads to macroscopic hydrogel contraction. The authors use stable polymer hydrogels, prepared through azide-alkyne crosslinking, containing surplus alkyne groups. γ-irradiation of these gels leads to more alkyne crosslinking, enabling controlled increase of crosslink...journal article 2021
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Breve, T. (author), Filius, M. (author), Weerdenburg, S. (author), van der Griend, Stefan J. (author), Groeneveld, T. (author), Denkova, A.G. (author), Eelkema, R. (author)Stimuli-responsive soft materials enable controlled release of loaded drug molecules and biomolecules. Controlled release of potent chemotherapeutic or immunotherapeutic agents is crucial to reduce unwanted side effects. In an effort to develop controlled release strategies that can be triggered by using Cerenkov luminescence, we have...journal article 2021
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Men, Y. (author), Breve, T. (author), Liu, H. (author), Denkova, A.G. (author), Eelkema, R. (author)We present a new light cleavable polymer containing o-nitrobenzene thioacetal groups in the main chain. By conjugation to a PEG block, we synthesized block copolymers capable of forming nanoparticles in aqueous solution. We studied drug encapsulation and release using the model drug Nile Red. Irradiation with UV-A light (365 nm) leads to...journal article 2021
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Bhardwaj, R. (author), Wolterbeek, H.T. (author), Denkova, A.G. (author), Serra Crespo, P. (author)A solid phase extraction based <sup>177m</sup>Lu-<sup>177</sup>Lu separation method has been investigated for its feasibility to be used in the radionuclide generator. The use of 2,2′,2”-(10-(2,6-dioxotetrahydro-2H-pyran-3-yl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid, (DOTAGA-anhydride) allowed grafting of DOTA (1,4,7,10...journal article 2020
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Moret, J.L.T.M. (author), Griffiths, Matthew B.E. (author), Frijns, Jeannine E.B.M. (author), Terpstra, B.E. (author), Wolterbeek, H.T. (author), Barry, Seán T. (author), Denkova, A.G. (author), van Ommen, J.R. (author)Atomic layer deposition (ALD) is a versatile gas phase coating technique that allows coating of complex structured materials, as well as high-surface area materials such as nanoparticles. In this work, ALD is used to deposit a lutetium oxide layer on TiO<sub>2</sub> nanoparticles (P25) in a fluidized bed reactor to produce particles for...journal article 2020
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Moret, J. L.T.M. (author), Alkemade, J. (author), Upcraft, T. M. (author), Paulssen, E. (author), Wolterbeek, H.T. (author), van Ommen, J.R. (author), Denkova, A.G. (author)New production routes for <sup>99</sup>Mo are steadily gaining importance. However, the obtained specific activity is much lower than currently produced by the fission of U-235. To be able to supply hospitals with <sup>99</sup>Mo/<sup>99m</sup>Tc generators with the desired activity, the adsorption capacity of the column material should be...journal article 2020
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Moret, J.L.T.M. (author), Hardens, T. A. (author), van Batenburg, O. (author), Wolterbeek, H.T. (author), van Ommen, J.R. (author), Denkova, A.G. (author)Cu is an important trace metal which plays a role in many biological processes. The radioisotope <sup>64</sup>Cu is often used to study such processes. Furthermore, <sup>64</sup>Cu finds applications in cancer diagnostics as well as therapy. For all of these applications <sup>64</sup>Cu having high specific activity is needed. <sup>64</sup>Cu...journal article 2020
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Zhang, W. (author), Liu, H. (author), van Schie, M.M.C.H. (author), Hagedoorn, P.L. (author), Alcalde, Miguel (author), Denkova, A.G. (author), Djanashvili, K. (author), Hollmann, F. (author)It is well-known that energy-rich radiation induces water splitting, eventually yielding hydrogen peroxide. Synthetic applications, however, are scarce and to the best of our knowledge, the combination of radioactivity with enzyme-catalysis has not been considered yet. Peroxygenases utilize H2O2 as an oxidant to promote highly selective...journal article 2020
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Roobol, Stefan J. (author), Hartjes, Thomas A. (author), Slotman, Johan A. (author), de Kruijff, R.M. (author), Kanaar, R. (author), Houtsmuller, A.B. (author), Denkova, A.G. (author), van Royen, M.E. (author), Essers, J. (author)Polymersomes have the potential to be applied in targeted alpha radionuclide therapy, while in addition preventing release of recoiling daughter isotopes. In this study, we investigated the cellular uptake, post uptake processing and intracellular localization of polymersomes. Methods: High-content microscopy was used to validate polymersome...journal article 2020
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Bhardwaj, R. (author), Wolterbeek, H.T. (author), Denkova, A.G. (author), Serra Crespo, P. (author)In order to determine the potential of <sup>177m</sup>Lu/<sup>177</sup>Lu radionuclide generator in <sup>177</sup>Lu production it is important to establish the technical needs that can lead to a clinically acceptable <sup>177</sup>Lu product quality. In this work, a model that includes all the processes and the parameters affecting the...journal article 2020
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de Kruijff, R.M. (author), Raavé, René (author), Kip, Annemarie (author), Molkenboer-Kuenen, Janneke (author), Roobol, Stefan J. (author), Essers, Jeroen (author), Heskamp, Sandra (author), Denkova, A.G. (author)The use of nanoparticles as tumor-targeting agents is steadily increasing, and the influence of nanoparticle characteristics such as size and stealthiness have been established for a large number of nanocarrier systems. However, not much is known about the impact of tumor presence on nanocarrier circulation times. This paper reports on the...journal article 2019
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Liu, H. (author), Carter, Philippe J.H. (author), Laan, A.C. (author), Eelkema, R. (author), Denkova, A.G. (author)A great number of fluorescent probes have been developed for detecting singlet oxygen (<sup>1</sup>O<sub>2</sub>), which is considered to be one of the most effective reactive oxygen species (ROS), especially in clinical applications. The commercially available fluorescent probe Singlet Oxygen Sensor Green (SOSG) is widely used due to its...journal article 2019
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de Kruijff, R.M. (author), Raavé, R. (author), Kip, A. (author), Molkenboer-Kuenen, J. (author), Morgenstern, A. (author), Bruchertseifer, F. (author), Heskamp, S. (author), Denkova, A.G. (author)Increasing attention is given to personalized tumour therapy, where α-emitters can potentially play an important role. Alpha particles are ideal for localized cell killing because of their high linear energy transfer and short ranges. However, upon the emission of an α particle the daughter nuclide experiences a recoil energy large enough to...journal article 2019
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Fauzia, R.P. (author), Denkova, A.G. (author), Djanashvili, K. (author)Radiation therapy has made tremendous progress in oncology over the last decades due to advances in engineering and physical sciences in combination with better biochemical, genetic and molecular understanding of this disease. Local delivery of optimal radiation dose to a tumor, while sparing healthy surrounding tissues, remains a great...journal article 2019
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Bikhezar, Fatima (author), de Kruijff, R.M. (author), van der Meer, A.J.G.M. (author), Torrelo Villa, G. (author), van der Pol, Susanne M.A. (author), Becerril Aragon, Gabriel (author), Gasol Garcia, Ana (author), Denkova, A.G. (author), Narayan, Ravi S. (author)Background and purpose: Glioblastoma multiforme (GBM) is the most aggressive subtype of malignant gliomas, with an average survival rate of 15 months after diagnosis. More than 90% of all GBMs have activating mutations in the MAPK/ERK pathway. Recently, we showed the allosteric MEK1/2 inhibitor binimetinib (MEK162) to inhibit cell...journal article 2019
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Bhardwaj, R. (author), Wolterbeek, H.T. (author), Denkova, A.G. (author), Serra Crespo, P. (author)Background In this work, a lutetium-177 (177Lu) production method based on the separation of nuclear isomers, 177mLu & 177Lu, is reported. The 177mLu-177Lu separation is performed by combining the use of DOTA & DOTA-labelled peptide (DOTATATE) and liquid-liquid extraction. Methods The 177mLu cations were complexed with DOTA &...journal article 2019
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Warman, J.M. (author), de Haas, M.P. (author), Luthjens, L.H. (author), Denkova, A.G. (author), Yao, T. (author)We review the development and application of an organic polymer-gel capable of producing fixed, three-dimensional fluorescent images of complex radiation fields. The gel consists for more than 99% of γ-ray-polymerized (~15% conversion) tertiary-butyl acrylate (TBA) containing ~100 ppm of a fluorogenic compound, e.g., maleimido-pyrene (MPy). The...journal article 2018
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Kouwenberg, J.J.M. (author), Wolterbeek, H.T. (author), Denkova, A.G. (author), bos, A.J.J. (author)Background: While alpha microdosimetry dates back a couple of decades, the effects of localized energy deposition of alpha particles are often still unclear since few comparative studies have been performed. Most modern alpha microdosimetry studies rely for large parts on simulations, which negatively impacts both the simplicity of the...journal article 2018
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Kouwenberg, J.J.M. (author), Kremers, G. J. (author), Slotman, J. A. (author), Wolterbeek, H.T. (author), Houtsmuller, A.B. (author), Denkova, A.G. (author), bos, A.J.J. (author)Structured illumination microscopy (SIM) for the imaging of alpha particle tracks in fluorescent nuclear track detectors (FNTD) was evaluated and compared to confocal laser scanning microscopy (CLSM). FNTDs were irradiated with an external alpha source and imaged using both methodologies. SIM imaging resulted in improved resolution, without...journal article 2018
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de Kruijff, R.M. (author), van der Meer, A.J.G.M. (author), Windmeijer, C.A.A. (author), Kouwenberg, J.J.M. (author), Morgenstern, A. (author), Bruchertseifer, F. (author), Sminia, P. (author), Denkova, A.G. (author)Alpha emitters have great potential in targeted tumour therapy, especially in destroying micrometastases, due to their high linear energy transfer (LET). To prevent toxicity caused by recoiled daughter atoms in healthy tissue, alpha emitters like <sup>225</sup>Ac can be encapsulated in polymeric nanocarriers (polymersomes), which are capable...journal article 2018