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Zheng, T. (author), Olson, D.G. (author), Tian, L. (author), Bomble, Y.J. (author), Himmel, M.E. (author), Lo, J. (author), Hon, S. (author), Shaw, A.J. (author), Van Dijken, J.P. (author), Lynd, L.R. (author)Clostridium thermocellum and Thermoanaerobacterium saccharolyticum are thermophilic bacteria that have been engineered to produce ethanol from the cellulose and hemicellulose fractions of biomass, respectively. Although engineered strains of T. saccharolyticum produce ethanol with a yield of 90% of the theoretical maximum, engineered strains of...journal article 2015
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Holwerda, E.K. (author), Thorne, P.G. (author), Olson, D.G. (author), Amador-Noguez, D. (author), Engle, N.L. (author), Tschaplinksi, T.J. (author), Van Dijken, J.P. (author), Lynd, L.R. (author)Background Clostridium thermocellum is a model thermophilic organism for the production of biofuels from lignocellulosic substrates. The majority of publications studying the physiology of this organism use substrate concentrations of ?10 g/L. However, industrially relevant concentrations of substrate start at 100 g/L carbohydrate, which...journal article 2014
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Zhou, J. (author), Olson, D.G. (author), Argyros, D.A. (author), Deng, Y. (author), Van Gulik, W.M. (author), Van Dijken, J.P. (author), Lynd, L.R. (author)Cofactor specificities of glycolytic enzymes in Clostridium thermocellum were studied with cellobiose-grown cells from batch cultures. Intracellular glucose was phosphorylated by glucokinase using GTP rather than ATP. Although phosphofructokinase typically uses ATP as a phosphoryl donor, we found only pyrophosphate (PPi)-linked activity....journal article 2013
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Zelle, R.M. (author), De Hulster, E. (author), Van Winden, W.A. (author), De Waard, P. (author), Dijkema, C. (author), Winkler, A.A. (author), Geertman, J.M. (author), Van Dijken, J.P. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)Malic acid is a potential biomass-derivable "building block" for chemical synthesis. Since wild-type Saccharomyces cerevisiae strains produce only low levels of malate, metabolic engineering is required to achieve efficient malate production with this yeast. A promising pathway for malate production from glucose proceeds via carboxylation of...journal article 2008
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Wisselink, H.W. (author), Toirkens, M.J. (author), Del Rosario Franco Berriel, M. (author), Winkler, A.A. (author), Van Dijken, J.P. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)For cost-effective and efficient ethanol production from lignocellulosic fractions of plant biomass, the conversion of not only major constituents, such as glucose and xylose, but also less predominant sugars, such as L-arabinose, is required. Wild-type strains of Saccharomyces cerevisiae, the organism used in industrial ethanol production,...journal article 2007
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Van Maris, A.J.A. (author), Winkler, A.A. (author), Kuyper, M. (author), De Laat, W.T. (author), Van Dijken, J.P. (author), Pronk, J.T. (author)Metabolic engineering of Saccharomyces cerevisiae for ethanol production from d-xylose, an abundant sugar in plant biomass hydrolysates, has been pursued vigorously for the past 15 years. Whereas wild-type S. cerevisiae cannot ferment d-xylose, the ketoisomer d-xylulose can be metabolised slowly. Conversion of d-xylose into d-xylulose is...journal article 2007
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- Van Maris, A.J.A. (author), Abbott, D.A. (author), Bellissimi, E. (author), Van den Brink, J. (author), Kuyper, M. (author), Lutik, M.A.H. (author), Wisselink, H.W. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author), Pronk, J.T. (author) journal article 2006
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Winkler, A.A. (author), Kuyper, S.M. (author), De Laat, W.T. (author), Van Dijken, J.P. (author), Pronk, J.T. (author)The present invention relates to further genetic modifications in eukaryotic host cells that have been transformed to express a xylose isomerase that confers the host cell the ability of isomerising xylose to xylulose. The further genetic modifications are aimed at improving the efficiency of xylose metabolism and include e.g. reduction of...patent 2006
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- Aguilera, J. (author), Van Dijken, J.P. (author), De Winde, J.H. (author), Pronk, J.T. (author) journal article 2005
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- van Maris, A.J. (author), Winkler, A.A. (author), Porro, D. (author), van Dijken, J.P. (author), Pronk, J.T. (author) journal article 2004
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- van Maris, A.J. (author), Geertman, J.M. (author), Vermeulen, A. (author), Groothuizen, M.K. (author), Winkler, A.A. (author), Piper, M.D. (author), van Dijken, J.P. (author), Pronk, J.T. (author) journal article 2004
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- van Maris, A.J. (author), Luttik, M.A. (author), Winkler, A.A. (author), van Dijken, J.P. (author), Pronk, J.T. (author) journal article 2003
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- Overkamp, K.M. (author), Bakker, B.M. (author), Kotter, P. (author), Luttik, M.A. (author), Van Dijken, J.P. (author), Pronk, J.T. (author) journal article 2002
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- van Dijken, J.P. (author), van Tuijl, A. (author), Luttik, M.A. (author), Middelhoven, W.J. (author), Pronk, J.T. (author) journal article 2002
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- Bakker, B.M. (author), Bro, C. (author), Kotter, P. (author), Luttik, M.A. (author), van Dijken, J.P. (author), Pronk, J.T. (author) journal article 2000
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- Overkamp, K.M. (author), Bakker, B.M. (author), Kotter, P. (author), van Tuijl, A. (author), de Vries, S. (author), van Dijken, J.P. (author), Pronk, J.T. (author) journal article 2000
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- Luttik, M.A. (author), Kotter, P. (author), Salomons, F.A. (author), van der Klei, I.J. (author), van Dijken, J.P. (author), Pronk, J.T. (author) journal article 2000
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- Van Hoek, P. (author), Van Dijken, J.P. (author), Pronk, J.T. (author) journal article 1998
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- van Hoek, P. (author), Flikweert, M.T. (author), van der Aart, Q.J.M. (author), Steensma, H.Y. (author), van Dijken, J.P. (author), Pronk, J.T. (author) journal article 1998
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- Flikweert, M.T. (author), van Dijken, J.P. (author), Pronk, J.T. (author) journal article 1997