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Metabolic engineering of Saccharomyces cerevisiae for production of carboxylic acids: Current status and challenges

Abbott, D.A. (author), Zelle, R.M. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)

To meet the demands of future generations for chemicals and energy and to reduce the environmental footprint of the chemical industry, alternatives for petrochemistry are required. Microbial conversion of renewable feedstocks has a huge potential for cleaner, sustainable industrial production of fuels and chemicals. Microbial production of...

journal article 2009

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Catalase Overexpression Reduces Lactic Acid-Induced Oxidative Stress in Saccharomyces cerevisiae

Abbott, D.A. (author), Suir, E. (author), Duong, G.H. (author), De Hulster, E. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)

Industrial production of lactic acid with the current pyruvate decarboxylase-negative Saccharomyces cerevisiae strains requires aeration to allow for respiratory generation of ATP to facilitate growth and, even under nongrowing conditions, cellular maintenance. In the current study, we observed an inhibition of aerobic growth in the presence of...

journal article 2009

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Exploring and dissecting genome-wide gene expression responses of Penicillium chrysogenum to phenylacetic acid consumption and penicillinG production

Harris, D.M. (author), Van der Krogt, Z.A. (author), Klaassen, P. (author), Raamsdonk, L.M. (author), Hage, S. (author), Van den Berg, M.A. (author), Bovenberg, R.A.L. (author), Pronk, J.T. (author), Daran, J.M. (author)

OA Fund TU Delft Background Since the discovery of the antibacterial activity of penicillin by Fleming 80 years ago, improvements of penicillin titer were essentially achieved by classical strain improvement through mutagenesis and screening. The recent sequencing of Penicillium chrysogenum strain Wisconsin1255-54 and the availability of...

journal article 2009

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Combinatorial effects of environmental parameters on transcriptional regulation in Saccharomyces cerevisiae: A quantitative analysis of a compendium of chemostat-based transcriptome data

Knijnenburg, T.A. (author), Daran, J.M.G. (author), Van den Broek, M.A. (author), Daran-Lapujade, P.A.S. (author), De Winde, J.H. (author), Pronk, J.T. (author), Reinders, M.J.T. (author), Wessels, L.F.A. (author)

OA Fund TU delft Background: Microorganisms adapt their transcriptome by integrating multiple chemical and physical signals from their environment. Shake-flask cultivation does not allow precise manipulation of individual culture parameters and therefore precludes a quantitative analysis of the (combinatorial) influence of these parameters on...

journal article 2009

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Metabolic engineering of Saccharomyces cerevisiae for production of carboxylic acids: current status and challenges

Abbott, DA (author), Zelle, RM (author), Pronk, J.T. (author), van Maris, A.J.A. (author)

journal article 2009

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Novel Evolutionary Engineering Approach for Accelerated Utilization of Glucose, Xylose, and Arabinose Mixtures by Engineered Saccharomyces cerevisiae Strains

Wisselink, H.W. (author), Toirkens, M.J. (author), Wu, Q. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)

Lignocellulosic feedstocks are thought to have great economic and environmental significance for future biotechnological production processes. For cost-effective and efficient industrial processes, complete and fast conversion of all sugars derived from these feedstocks is required. Hence, simultaneous or fast sequential fermentation of sugars...

journal article 2008

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Malic Acid Production by Saccharomyces cerevisiae: Engineering of Pyruvate Carboxylation, Oxaloacetate Reduction, and Malate Export

Zelle, R.M. (author), De Hulster, E. (author), Van Winden, W.A. (author), De Waard, P. (author), Dijkema, C. (author), Winkler, A.A. (author), Geertman, J.M. (author), Van Dijken, J.P. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)

Malic acid is a potential biomass-derivable "building block" for chemical synthesis. Since wild-type Saccharomyces cerevisiae strains produce only low levels of malate, metabolic engineering is required to achieve efficient malate production with this yeast. A promising pathway for malate production from glucose proceeds via carboxylation of...

journal article 2008

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The Ehrlich Pathway for Fusel Alcohol Production: A Century of Research on Saccharomyces cerevisiae Metabolism

Hazelwood, L.A. (author), Daran, J.M. (author), Van Maris, A.J.A. (author), Pronk, J.T. (author), Dickinson, J.R. (author)

journal article 2008

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Transcription factor control of growth rate dependent genes in Saccharomyces cerevisiae: A three factor design

Fazio, A. (author), Jewett, M.C. (author), Daran-Lapujade, P. (author), Mustacchi, R. (author), Usaite, R. (author), Pronk, J.T. (author), Workman, C.T. (author), Nielsen, J. (author)

journal article 2008

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New insights into the Saccharomyces cerevisiae fermentation switch: Dynamic transcriptional response to anaerobicity and glucose-excess

Van Den Brink, J. (author), Daran-Lapujade, P. (author), Pronk, J.T. (author), De Winde, J.H. (author)

OA Fund

journal article 2008

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Alleviation of feedback inhibition in Saccharomyces cerevisiae aromatic amino acid biosynthesis: Quantification of metabolic impact

Luttik, M.A.H. (author), Vuralhan, Z (author), Suir, E (author), Braus, G.H. (author), Pronk, J.T. (author), Daran, J.G. (author)

A quantitative analysis of the impact of feedback inhibition on aromatic amino acid biosynthesis was performed in chemostat cultures of Saccharomyces cerevisiae. Introduction of a tyrosine-insensitive allele of ARO4 (encoding 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase) caused a three-fold increase of intracellular phenylalanine and...

journal article 2008

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Acclimation of Saccharomyces cerevisiae to Low Temperature: A Chemostat-based Transcriptome Analysis

Tai, S.L. (author), Daran-Lapujade, P. (author), Walsh, M.C. (author), Pronk, J.T. (author), Daran, J.M. (author)

Effects of suboptimal temperatures on transcriptional regulation in yeast have been extensively studied in batch cultures. To eliminate indirect effects of specific growth rates that are inherent to batch-cultivation studies, genome-wide transcriptional responses to low temperatures were analyzed in steady-state chemostats, grown at a fixed...

journal article 2007

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The fluxes through glycolytic enzymes in Saccharomyces cerevisiae are predominantly regulated at posttranscriptional levels

Daran, J.M. (author), Bakker, B.M. (author), Pronk, J.T. (author), Westerhoff, H.V. (author), De Winde, J.H. (author), Heck, A.J.R. (author), Slijper, M. (author), De Groot, M.J.L. (author), Luttik, M.A.H. (author), Van Gulik, W.M. (author), Rossell, S. (author), Daran-Lapujade, P. (author)

Metabolic fluxes may be regulated ‘‘hierarchically,’’ e.g., by changes of gene expression that adjust enzyme capacities (Vmax) and/or ‘‘metabolically’’ by interactions of enzymes with substrates, products, or allosteric effectors. In the present study, a method is developed to dissect the hierarchical regulation into contributions by...

journal article 2007

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Engineering of Saccharomyces cerevisiae for Efficient Anaerobic Alcoholic Fermentation of L-Arabinose

Wisselink, H.W. (author), Toirkens, M.J. (author), Del Rosario Franco Berriel, M. (author), Winkler, A.A. (author), Van Dijken, J.P. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)

For cost-effective and efficient ethanol production from lignocellulosic fractions of plant biomass, the conversion of not only major constituents, such as glucose and xylose, but also less predominant sugars, such as L-arabinose, is required. Wild-type strains of Saccharomyces cerevisiae, the organism used in industrial ethanol production,...

journal article 2007

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Generic and specific transcriptional responses to different weak organic acids in anaerobic chemostat cultures of Saccharomyces cerevisiae.

Abbott, D.A. (author), Knijnenburg, T.A. (author), De Poorter, L.M. (author), Reinders, M.J. (author), Pronk, J.T. (author), Van Maris, A.J. (author)

Transcriptional responses to four weak organic acids (benzoate, sorbate, acetate and propionate) were investigated in anaerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae. To enable quantitative comparison of the responses to the acids, their concentrations were chosen such that they caused a 50% decrease of the biomass...

journal article 2007

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Development of Efficient Xylose Fermentation in Saccharomyces cerevisiae: Xylose Isomerase as a Key Component

Van Maris, A.J.A. (author), Winkler, A.A. (author), Kuyper, M. (author), De Laat, W.T. (author), Van Dijken, J.P. (author), Pronk, J.T. (author)

Metabolic engineering of Saccharomyces cerevisiae for ethanol production from d-xylose, an abundant sugar in plant biomass hydrolysates, has been pursued vigorously for the past 15 years. Whereas wild-type S. cerevisiae cannot ferment d-xylose, the ketoisomer d-xylulose can be metabolised slowly. Conversion of d-xylose into d-xylulose is...

journal article 2007

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Control of the Glycolytic Flux in Saccharomyces cerevisiae Grown at Low Temperature: A multi-level analysis in anaerobic chemostat cultures

Tai, S.L. (author), Daran-Lapujade, P. (author), Luttik, M.A. (author), Walsh, M.C. (author), Diderich, J.A. (author), Krijger, G.C. (author), Van Gulik, W.M. (author), Pronk, J.T. (author), Daran, J.M. (author)

journal article 2007

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Exploiting combinatorial cultivation conditions to infer transcriptional regulation

Knijnenburg, T.A. (author), De Winde, J.H. (author), Daran, J.M. (author), Daran-Lapujade, P. (author), Pronk, J.T. (author), Reinders, M.J.T. (author), Wessels, L.F.A. (author)

journal article 2007

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Exploiting combinatorial cultivation conditions to infer transcriptional regulation

Knijnenburg, T.A. (author), De Winde, J.H. (author), Daran, J.M. (author), Daran-Lapujade, P. (author), Pronk, J.T. (author), Reinders, M.J.T. (author), Wessels, L.F.A. (author)

journal article 2007

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Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: Current status

Van Maris, A.J.A. (author), Abbott, D.A. (author), Bellissimi, E. (author), Van den Brink, J. (author), Kuyper, M. (author), Lutik, M.A.H. (author), Wisselink, H.W. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author), Pronk, J.T. (author)

journal article 2006