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Hung, S. (author), Cnossen, J.P. (author), Fan, D. (author), Siemons, Marijn (author), Jurriens, Daphne (author), Grußmayer, K.S. (author), Soloviev, O.A. (author), Kapitein, Lukas C. (author), Smith, C.S. (author)High-NA light sheet illumination can improve the resolution of single-molecule localization microscopy (SMLM) by reducing the background fluorescence. These approaches currently require custom-made sample holders or additional specialized objectives, which makes the sample mounting or the optical system complex and therefore reduces the...journal article 2022
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Cnossen, J.P. (author), Hinsdale, T.A. (author), Thorsen, R.Ø. (author), Siemons, Marijn (author), Schueder, Florian (author), Jungmann, Ralf (author), Smith, C.S. (author), Rieger, B. (author), Stallinga, S. (author)MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold...journal article 2020
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Siemons, M.E. (author), Hulleman, C.N. (author), Thorsen, R.Ø. (author), Smith, C.S. (author), Stallinga, S. (author)Point spread function (PSF) engineering is used in single emitter localization to measure the emitter position in 3D and possibly other parameters such as the emission color or dipole orientation as well. Advanced PSF models such as spline fits to experimental PSFs or the vectorial PSF model can be used in the corresponding localization...journal article 2018