Organ-on-chip (OoC) technology has transformed biomedical research by providing a platform to simulate physiological conditions for drug development and disease modeling. Incubator-free OoC systems offer notable advantages over traditional approaches, including enhanced adaptability and impermeability to gases. Nevertheless, achieving precise oxygen regulation remains a challenge in such systems. This thesis investigates the application of femtosecond (Fs) laser ablation to fabricate a glass microfluidic gas exchange system for accurate oxygen regulation in incubator-free OoCs.
Through successful engraving of fluidic channels and integration of an off-the-shelf optical oxygen sensor, this study highlights the efficacy of Fs laser technology in the rapid prototyping of intricate glass microfluidic devices. Despite encountering challenges such as dimensional losses and debris clogging, the study presents a functional gas exchanger prototype. Future research directions include optimization efforts, addressing issues like gas permeation through connectors, and testing under physiological conditions to further advance OoC technology.

Micropumps are essential for providing controlled fluid dynamics in Organ-on-a-Chip (OoC) devices. Additive manufacturing builds up prototypes in several hours with a free-geometry advantage. Therefore, this master's thesis investigates the utilization of additive manufacturing to produce a micropump with a flowrate in the range of several $\mu l/min$ for OoC applications. A ball valve-based piezoelectric micropump was fabricated with a mSLA 3D printer. This micropump generates unidirectional flow through the reciprocating motion of the piezoelectric actuator and the movement of a ball within the conical channel. The virtual mass due to the inertia of the fluid inside the chamber shifts the resonance frequency of the piezoelectric actuator from the 1600 Hz to 43 Hz. The maximum flow rate of $26.5 \mu l/min$ was generated when the applied sinusoidal voltage was 240 Vpp at 5Hz and the maximum back pressure of 36.5 mbar was obtained under this power supply. These results confirm that additive manufacturing provides a promising option for miniature pump manufacturing.