Single‐Molecule Switching

Single‐Molecule Switching: Fluorescence Polarization Control for On–Off Switching of Single Molecules at Cryogenic Temperatures

Hulleman, C.N. (TU Delft ImPhys/Quantitative Imaging)
Huisman, Maximiliaan (University of Massachusetts Medical School)
Moerland, R.J. (TU Delft ImPhys/Quantitative Imaging)
Grünwald, David (RNA Therapeutics Institute, University of Massachusetts Medical School)
Stallinga, S. (TU Delft ImPhys/Imaging Physics)
Rieger, B. (TU Delft ImPhys/Quantitative Imaging)

ImPhys/Imaging Physics

2018

In article number 1700323, a sparsity‐inducing scheme based on widefield stimulated emission depletion, which requires stringent control of the polarization state of both the excitation and depletion laser, is introduced by Bernd Rieger and co‐workers for superresolution fluorescence microscopy. The ideal state involves excitation and depletion lasers with linear polarizations, orthogonally oriented in the sample plane. This state is obtained from linearly polarized light from the laser, which is made elliptically polarized before reflecting off the dichroic mirror.

http://resolver.tudelft.nl/uuid:3a889c8d-6bce-4dd2-a4e8-c3170052c4ff

https://doi.org/10.1002/smtd.201870044

2019-03-14

2366-9608

SMALL METHODS, 2 (9)

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

Institutional Repository

journal article

© 2018 C.N. Hulleman, Maximiliaan Huisman, R.J. Moerland, David Grünwald, S. Stallinga, B. Rieger