Integrated attenuated total reflection – Fourier transform infrared spectroscopy (ATR-FTIR) – Electrochemical impedance spectroscopy (EIS) measurements were used to simultaneously follow chemisorption mechanisms of organic inhibitors as well as their corrosion inhibition efficiency towards magnesium based substrates. Four carboxylic compounds, i.e. 2,5-pyridinedicarboxylic acid (PDC), 3-methylsalicylic acid (MSA), sodium salicylate (SS) and fumaric acid (FA), were selected based on their promising inhibiting capacities and were all shown to chemisorb at the MgO/Mg(OH)₂ surface by carboxylate bond formation. Orientation analysis using polarized infrared light showed that carboxylate bonds established using aliphatic carboxylate compound aligned perpendicular to the magnesium surface, whereas carboxylate bonds with aromatic compounds were oriented in plane with the magnesium surface. This different orientation is associated to the involvement of π-interactions in the MgO/Mg(OH)₂ – aromatic carboxylate adsorption. Additionally, DFT calculations revealed that the addition of hetero-atoms (i.e. N or OH) in the molecular structure contributes to increased adsorption energies, indicating that next to carboxylate groups also these hetero-atoms are involved in interfacial interactions. Integrating the ATR-FTIR setup with an electrochemical cell allowing for simultaneous EIS measurements lead to two surface phenomena determining the inhibition efficiency. Surface hydroxylation processes on one hand forming a MgO/Mg(OH)₂ layer on one hand, and the chemisorption of carboxylate compounds on the other hand. The inhibition efficiency was found to increase in following order: FA < PDC < MSA and was mainly associated to the formation of a MgO/Mg(OH)₂ layer. SS was shown to act as a corrosion accelerator rather than a corrosion inhibitor. Despite its high sensitivity for water, both surface processes could be followed in situ by means of ATR-FTIR. Simultaneously, protective properties of the formed films could be quantified by means of EIS. Consequently, integrated ATR-FTIR – EIS methodology has shown to be highly valuable for gaining in-situ insights in the inhibition mechanism, while quantifying the inhibition efficiency. This was even possible for highly active metal substrate as magnesium, although further developments are suggested if one aims to quantify electrochemical constants related to corrosion and other surface processes measured at the low frequencies (i.e. < 1 Hz).

The molecular configuration and chemistry at the zinc/zinc oxide-polyester interface were studied by using two complementary spectroscopic techniques: attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and sum-frequency generation (SFG) spectroscopy. It was shown that ATR-FTIR should be considered as a (3D) interphase-sensitive technique with probing depths of 250-400 nm in the headgroup region (2000-1200 cm^-1). On the other hand, SFG is known to be a (2D) interface-sensitive technique. The ATR-FTIR measurements showed that carboxylate groups are formed within the near-interface region of the polyester phase. SFG measurements showed that the carboxylic acid groups are stable at the polymer-zinc/zinc oxide interface. In addition, in situ ATR-FTIR and SFG measurements have been conducted when exposing the polyester-zinc/zinc oxide system to D₂O. The exposure to D₂O is observed to lead to an additional conversion of ester and carboxylic acid groups to carboxylate groups. The comparison of the SFG and ATR-FTIR measurements shows that this conversion occurs much slower at the polyester-zinc/zinc oxide interface than in the bulk of the polyester. Finally, the strengths and limitations as well as the complementarity of both techniques are discussed.

The effect of zirconium-based conversion of thermally vaporized zinc, aluminium and magnesium on the chemisorption of dimethylsuccinate was studied using attenuated total reflection – Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS) analysis. Two competing chemisorption mechanisms contribute to interfacial bond formation. Hydrogen interactions on one hand were shown to occur between metal hydroxides and non-hydrolysed ester-groups of the molecule. On the other hand, both native and zirconium-treated substrates were shown to form interfacial carboxylate bonds with dimethylsuccinate, evidencing their capability of hydrolysing ester groups towards more reactive acid groups. Both interactions were shown to correlate to the metal oxide acid-base properties.

The bonding properties of zirconium- and titanium-based conversion coatings were evaluated using model conversion solutions of H₂ZrF₆ and H₂TiF₆ with addition of various organic additives (PAA, PVA, PVP). Macroscopic testing techniques such as contact angle and pull-off adhesion measurements were performed on galvanized steel sheets. Complementary to this, molecular studies were performed on model zinc substrates using ATR-FTIR in the Kretschmann configuration. The macroscopic and molecular approaches showed a good correlation demonstrating ATR-FTIR in the Kretschmann configuration to be a valuable tool to gain fundamental insights in metal oxide-polymer interfacial phenomena. Zirconium-treated galvanized steel substrates were shown to have a higher bonding affinity for the polyester coil coat primer than titanium-treated galvanized steel substrates. The presence of organic additives did not further improve the bonding properties. Yet, organic additives initially improved the interfacial stability of titanium-treated substrates. However, on the long term, organic additives are shown to be detrimental for polyester coil coat adhesion. This adverse effect of organic additives on the long term was assigned to its selective dissolution during immersion and was most pronounced for titanium-treatments. The limited effect of organic additives in case of zirconium-treatments was attributed to the higher portion of chemical interfacial bonds, as well as its tendency for crosslinking reactions causing entanglement of polymeric compounds in the zirconium oxide structure.

In this work, in-situ ATR-FTIR in the Kretschmann configuration is proposed as an interfacial sensitive technique able to probe molecular processes at the buried interface of an industrial relevant polyester primer. Zinc, aluminium and magnesium oxide were used to represent oxides present at galvanized steel sheets used in coil coating. Two competing interactions with polyester resin and melamine-based crosslinker were shown to take place at metal hydroxide sites. This highlights the increased complexity of interfacial phenomena at metal–paint interfaces. Furthermore, in-situ ATR-FTIR was performed in deuterated water (D₂O) to study the evolution of interfacial carboxylate bond degradation, without overlap of dominant water signals. For the first time, interfacial bond formation of paints and its degradation in an aqueous environment is studied in-situ. It is shown that the introduction of D₂O at the interface initially increases the amount of interfacial carboxylate bonds, whereas upon longer exposure times bond degradation occurs. Significant delay of interfacial bond degradation on hexafluorozirconic acid treated oxides indicate successful stabilization of the metal-polymer interface by zirconium-based conversion coatings. Consequently, in-situ ATR-FTIR is able to demonstrate improved interfacial stability due to zirconium-based treatment in real-time and on a molecular level.

A common approach to investigate chemical interactions at the polymer/metal oxide interface is by monitoring ultrathin polymer films onto a metal oxide substrate by a variety of surface analysis techniques. The deposition of this nanometer-thin overlayer is frequently carried out by reactive adsorption from dilute polymer solutions. However, the influence of the solvent on the metal oxide chemistry is seldom taken into account in interface studies. The overall amount of available adsorption sites on the metal oxide surface might decrease due to competing adsorption of the solvent and the polymer adsorbate. Therefore, in this work, the adsorption of a common organic solvent (methanol) onto a physical vapor-deposited aluminum oxide surface is monitored in situ by an integrated attenuated total reflectance Fourier transform infrared spectroscopy in the Kretschmann geometry and odd random phase multisine electrochemical impedance spectroscopy system. It is shown that methanol immediately physisorbs onto the aluminum oxide surface and replaces the initial adventitious carbon layer. This process is followed by methanol chemisorbing onto the oxide surface to form methoxide species at the liquid/solid interface. Additionally, chemisorption is validated ex situ by X-ray photoelectron spectroscopy.

Additively manufactured (AM) topologically ordered porous metallic biomaterials with the proper biodegradation profile offer a unique combination of properties ideal for bone regeneration. These include a fully interconnected porous structure, bone-mimicking mechanical properties, and the possibility of fully regenerating bony defects. Most of such biomaterials are, however, based on magnesium and, thus, degrade too fast. Here, we present the first report on topologically ordered porous iron made by Direct Metal Printing (DMP). The topological design was based on a repetitive diamond unit cell. We conducted a comprehensive study on the in vitro biodegradation behavior (up to 28 days), electrochemical performance, time-dependent mechanical properties, and biocompatibility of the scaffolds. The mechanical properties of AM porous iron (E = 1600–1800 MPa) were still within the range of the values reported for trabecular bone after 28 days of biodegradation. Electrochemical tests showed up to ≈12 times higher rates of biodegradation for AM porous iron as compared to that of cold-rolled (CR) iron, while only 3.1% of weight loss was measured after 4 weeks of immersion tests. The biodegradation mechanisms were found to be topology-dependent and different between the periphery and central parts of the scaffolds. While direct contact between MG-63 cells and scaffolds revealed substantial and almost instant cytotoxicity in static cell culture, as compared to Ti-6Al-4V, the cytocompatibility according to ISO 10993 was reasonable in in vitro assays for up to 72 h. This study shows how DMP could be used to increase the surface area and decrease the grain sizes of topologically ordered porous metallic biomaterials made from metals that are usually considered to degrade too slowly (e.g., iron), opening up many new opportunities for the development of biodegradable metallic biomaterials. Statement of Significance: Biodegradation in general and proper biodegradation profile in particular are perhaps the most important requirements that additively manufactured (AM) topologically ordered porous metallic biomaterials should offer in order to become the ideal biomaterial for bone regeneration. Currently, most biodegradable metallic biomaterials are based on magnesium, which degrade fast with gas generation. Here, we present the first report on topologically ordered porous iron made by Direct Metal Printing (DMP). We also conducted a comprehensive study on the biodegradation behavior, electrochemical performance, biocompatibility, and the time evolution of the mechanical properties of the implants. We show that these implants possess bone-mimicking mechanical properties, accelerated degradation rate, and reasonable cytocompatibility, opening up many new opportunities for the development of iron-based biodegradable materials.

Zirconium-based conversion treatment of zinc, aluminium and magnesium oxides have been studied in-situ using ATR-FTIR in a Kretschmann geometry. This set-up was coupled to an electrochemical cell, which allowed to obtain chemical and electrochemical information simultaneously as a function of conversion time. This elucidated the strong relation between physico-chemical surface properties and zirconium-based conversion kinetics. Whereas the surface hydroxyl density of zinc and aluminium increased during conversion, magnesium (hydr)oxide was shown to dissolve in the acid solution. Due to this dissolution, strong surface alkalization can be expected, explaining the rapid conversion kinetics. AES depth profiling was used to determine the final oxide thickness and elemental composition. This confirmed that magnesium is most active and forms a zirconium oxide layer approximately 10 times thicker than zinc. On the other hand, the presence of zirconium oxide on aluminium is very low and can be considered as not fully covering the metal oxide. Additionally, the converted oxide chemistry was related to the bonding mechanisms of amide functionalized molecules using ATR-FTIR and XPS. It was shown that inclusion of zirconium altered the acid-base properties, increasing the substrate proton donating capabilities in case of magnesium oxide and increasing hydrogen bonding and Bronsted interactions due to increased surface hydroxide fractions on zinc and aluminium substrates.

An ideal bone substituting material should be bone-mimicking in terms of mechanical properties, present a precisely controlled and fully interconnected porous structure, and degrade in the human body to allow for full regeneration of large bony defects. However, simultaneously satisfying all these three requirements has so far been highly challenging. Here we present topologically ordered porous magnesium (WE43) scaffolds based on the diamond unit cell that were fabricated by selective laser melting (SLM) and satisfy all the requirements. We studied the in vitro biodegradation behavior (up to 4 weeks), mechanical properties and biocompatibility of the developed scaffolds. The mechanical properties of the AM porous WE43 (E = 700-800 MPa) scaffolds were found to fall into the range of the values reported for trabecular bone even after 4 weeks of biodegradation. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), electrochemical tests and μCT revealed a unique biodegradation mechanism that started with uniform corrosion, followed by localized corrosion, particularly in the center of the scaffolds. Biocompatibility tests performed up to 72 h showed level 0 cytotoxicity (according to ISO 10993-5 and -12), except for one time point (i.e., 24 h). Intimate contact between cells (MG-63) and the scaffolds was also observed in SEM images. The study shows for the first time that AM of porous Mg may provide distinct possibilities to adjust biodegradation profile through topological design and open up unprecedented opportunities to develop multifunctional bone substituting materials that mimic bone properties and enable full regeneration of critical-size load-bearing bony defects. Statement of Significance: The ideal biomaterials for bone tissue regeneration should be bone-mimicking in terms of mechanical properties, present a fully interconnected porous structure, and exhibit a specific biodegradation behavior to enable full regeneration of bony defects. Recent advances in additive manufacturing have resulted in biomaterials that satisfy the first two requirements but simultaneously satisfying the third requirement has proven challenging so far. Here we present additively manufactured porous magnesium structures that have the potential to satisfy all above-mentioned requirements. Even after 4 weeks of biodegradation, the mechanical properties of the porous structures were found to be within those reported for native bone. Moreover, our comprehensive electrochemical, mechanical, topological, and biological study revealed a unique biodegradation behavior and the limited cytotoxicity of the developed biomaterials.

Probing initial interactions at the interface of hybrid systems under humid conditions has the potential to reveal the local chemical environment at solid/solid interfaces under real-world, technologically relevant conditions. Here, we show that ambient pressure X-ray photoelectron spectroscopy (APXPS) with a conventional X-ray source can be used to study the effects of water exposure on the interaction of a nanometer-thin polyacrylic acid (PAA) layer with a native aluminum oxide surface. The formation of a carboxylate ionic bond at the interface is characterized both with APXPS and in situ attenuated total reflectance Fourier transform infrared spectroscopy in the Kretschmann geometry (ATR-FTIR Kretschmann). When water is dosed in the APXPS chamber up to 5 Torr (∼28% relative humidity), an increase in the amount of ionic bonds at the interface is observed. To confirm our APXPS interpretation, complementary ATR-FTIR Kretschmann experiments on a similar model system, which is exposed to an aqueous electrolyte, are conducted. These spectra demonstrate that water leads to an increased wet adhesion through increased ionic bond formation.