Logistics plays a crucial role in modern society, particularly in densely populated urban areas, facilitating the transportation of goods. Last-mile e-commerce deliveries are emissions-intensive, contributing significantly to CO2 levels and traffic congestion. Addressing this challenge requires systemic changes in last-mile delivery ecosystems. Based on this observation, in alignment with the EU decarbonisation goals, the URBANE project (GA101069782) aims to promote the adoption of sustainable and environmentally friendly last-mile delivery solutions by introducing a collaborative layered “Platform as a Service” (PaaS) paradigm. The initiative focuses on establishing Physical Internet (PI) inspired interventions combined with the implementation of innovative tools, such as agent-based and AI models, employing a Digital Twin platform addressing the operational and strategic planning challenges of city logistics networks. A multi-factorial impact assessment radar further enhances the evaluation of the PI interventions’ effectiveness. The platform fosters collaboration among urban logistics stakeholders governed through “green” smart contracts, addressing security and privacy concerns by using a blockchain infrastructure and digital IDs, creating a trustworthy system for collaboration. The paper showcases the applicability of the URBANE Innovation Transferability Platform in designing, measuring, testing, and validating targeted logistics interventions in Lighthouse Living Labs. Cities and logistic operators receive suggestions for informed data-driven decision-making coupled with integrated and transferable applications that can be standardised and structured, aligned with the targets set in a citie’s Sustainable Urban Logistics Plan (SULP).