Imaging mass spectrometry (IMS) enables untargeted, highly multiplexed mapping of molecular species in biological tissue with unparalleled chemical specificity and sensitivity. However, most IMS platforms lack microscopy-level spatial resolution and cellular morphological contrast, necessitating subsequent histochemical staining, microscopic imaging, and advanced image registration to correlate/link molecular distributions with specific tissue features and cell types. We present a diffusion model-based virtual histological staining approach that enhances spatial resolution and digitally introduces cellular morphological contrast into mass spectrometry images of label-free human tissue. Blind testing on human kidney tissue demonstrated that the virtually stained images of label-free samples closely match their histochemically stained counterparts (with periodic acid-Schiff staining), showing high concordance in identifying key renal pathology structures despite using IMS data with 10-fold larger pixel size. Additionally, our approach uses optimized noise sampling during the diffusion model's inference to achieve reliable and repeatable virtual staining. We believe this virtual staining method will open avenues for IMS-based biomedical research.

One of the major challenges in advancing polymer-inorganic hybrid solid electrolytes (HSEs) lies in comprehending and controlling their internal structure. In addition, the intricate interplay between multiple phases further complicates efforts to establish the structure-property relationships. In this study, by introducing a multifunctional LiI additive to an HSE compromising of polyethylene oxide (PEO) polymeric electrolyte and the fast lithium-ion conductor Li₆PS₅Cl, the relationship between the bulk and interface structure and ascertaining their impact on lithium-ion dynamics within the HSE is disentangled. Using multidimensional solid-state nuclear magnetic resonance, we find that the addition of LiI stabilizes the internal interfaces and enhances lithium-ion mobility. A kinetically stable solid-electrolyte interphase is formed at the lithium-metal anode, increasing the critical current density to 1.3 mA cm⁻², and enabling long-term stable cycling of lithium symmetric cells (>1200 h). This work sheds light on tailoring the structure of HSEs to improve their conductivity and stability for enabling all-solid-state lithium-metal batteries.

All-solid-state batteries receive ample attention due to their promising safety characteristics and energy density. The latter holds true if they are compatible with next-generation high-capacity anodes, but most highly ion-conductive solid electrolytes decompose at low operating potentials, leading to lithium loss and increased cell resistances. Here the dynamic stability of solid electrolytes that can improve all-solid-state battery performance is demonstrated. Halide electrolytes Li₃YCl₃Br₃ and Li₂ZrCl₆, considered unstable at low potentials, are found to exhibit structurally reversible redox activity beyond their electrochemical stability windows, increasing compatibility with anodes and contributing to capacity without compromising ionic conductivity. The benefit of this dynamic stability window is demonstrated with cost-effective red phosphorus anodes, resulting in high reversible capacities (2,308 mAh g⁻¹), high rate capacity retention (1,024 mAh g⁻¹ at 7.75 mA cm⁻²) and extended cycle life (61% retention after 1,780 cycles). Furthermore, high areal capacity (7.65 mAh cm⁻²) and stability (70% retention after 1,000 cycles) are achieved for halide-based full cells with red phosphorous anodes. The beneficial redox activity of halide electrolytes greatly expands their application scenarios and suggests valuable battery design principles to enhance performance.