Benthic diatoms are sensitive indicators of environmental conditions at the seabed. In this study, benthic diatom communities at two brine outfall sites of reverse osmosis (RO) seawater desalination plants in Larnaca and Dhekelia, Cyprus, were investigated using a classical, microscopy-based approach and environmental DNA metabarcoding. In general, the diversity of diatoms measured by both methods (microscopy and eDNA metabarcoding), increased by distance from the brine discharge. Increased TOC and nutrient enrichment at brine outfalls contributed to decreased diatom diversity at the Larnaca outfalls, but the diatom diversity at Dhekelia was not driven by abiotic factors. The diatom communities at the outfalls were shown to be distinct and showed temporal variation across the sampling seasons with eDNA metabarcoding, but this was the case only for Dhekelia with microscopy. The results highlight the effect of local biogeography and different brine mixing methods on diatom diversity. The results revealed that conventional morphological methods and eDNA metabarcoding rarely leads to similar conclusions. However, the complementary results emphasise that more information can be derived when combining the methods for biodiversity impact assessments.

Valuable and rare materials in seawater brine are often discarded during desalination. However, there is an increasing focus on recovering these resources, due to the economic and environmental opportunities it can bring. Despite this shift, current Sustainability Assessments (SA) in desalination overlook the brine handling and social dimensions, and brine treatment assessments remain centered on techno-economic dimensions. This work proposes a comprehensive framework for the SA of integrated desalination and resource recovery options, focusing on recovering valuable materials from brine. The framework not only evaluates pre-defined systems but supports the identification of system features of interest, such as products to assess and technologies to include, as well as the transparent selection of indicators, considering specific contexts. To develop this framework, a review of the literature on SA in desalination and brine treatment systems was conducted. Looking at the identified gaps, we synthesized the findings and key messages and proposed the integration of Multi-Criteria Analysis and Value-Sensitive Design in the decision-making process. This allows stakeholders to be involved and incorporates their values at different stages of the assessment, making it distinct from traditional SA methods. This framework offers structured guidance to stakeholders on how to carry out qualitative and quantitative assessments while ensuring transparency in the assessment process.

Seawater desalination has become an essential part of the drinking water supply of many arid countries in the world – including Cyprus and the wider Mediterranean – Middle Eastern region. In this study, the microinvertebrate fauna near the outfall sites of 2 seawater desalination plants, Dhekelia and Larnaca, in Cyprus was assessed by microscopy-based classical taxonomy and eDNA metabarcoding. The results show impact of desalination brine discharge on marine benthic communities. The community structure and the impact of abiotic factors was more prominent in the sediments than the water, and the alpha diversity results from metabarcoding were consistent with the results from microscopy but differed taxonomically.

Water treatment facilities are bound to incorporate resource recovery in the near future, necessitating novel economic assessments that capture the full economic potential of these systems. This study evaluates three cost calculation methods—Non-allocation, Economic allocation, and Dual allocation— to improve the accuracy of the Levelized Cost for multi-product desalination and brine treatment plants. The methods were tested across three technical scenarios: Sc1) maximum water recovery, Sc2) integrated desalination with brine treatment for resource recovery and Sc3) electricity-based desalination for chemical recovery. Results reveal that the traditional Non-allocation method tends to overestimate production costs by uniformly applying fixed costs across products, leading to inflated levelized costs. The Economic allocation approach reduces the levelized costs of water and other recovered products by up to 81 %, enhancing competitiveness with conventional production methods. The Dual allocation approach is most effective for recovered salts and chemicals, ensuring fair cost distribution and fostering competitiveness with linear systems. Sc2 is the most economically feasible under both novel approaches due to its balanced mix of high-value products and moderate operational costs. These findings suggest that cost calculation methods should align with plant objectives: Economic allocation for scenarios prioritizing water recovery and Dual allocation for maximizing the value of salts and chemicals. This study provides a foundation for tailored economic assessments and guides plant design and investment decisions.

The availability of water is still one of the most important factors affecting the sustainable growth of a country. Although many countries have free access to an inexhaustible source of water, the sea, this source cannot be used for human purposes as it is. To face this problem, desalination has been proposed for freshwater production but the generation of a waste brine effluent poses some issues of actual sustainability. In this work, the operation results of a Multiple Effect Distillation (MED) demo plant, designed for stable operation at high brine concentrations and operated as a brine concentrator, are presented. To this purpose, the integration with NanoFiltration (NF) has been implemented to minimize scaling risks, by removing bivalent ions from the feed stream. The 2-effects MED pilot unit, with a capacity of 1.7 m³/h, has been installed as part of the treatment chain of the WATER-MINING project, within the premises of the power station of the island of Lampedusa (Sicily, Italy) and is fully powered by waste heat at 70–80 °C from diesel engines. A vapor temperature of 40–50 °C allowed a perfect coupling with the low temperature waste heat source, demonstrating the possibility to produce distilled water with a conductivity between 15 and 25 μs/cm. Among the several operating conditions investigated, a recovery ratio above 80 % has been achieved and an effluent brine conductivity of 240 mS/cm was produced, very close to saturation in NaCl, thus being excellent for food-grade sea salt production in evaporative ponds. For the first time, it has been demonstrated on a pilot scale how a MED unit, supplied with waste heat, can be used efficiently as a brine concentrator, obtaining a brine concentration 8 times higher than the input concentration without any scaling problem.

Water and energy are two strategic drivers of sustainable development, intimately interlaced and vital for a secure future of humanity. Given that water resources are limited, whereas global population and energy demand are exponentially growing, the competitive balance between these resources, referred to as the water-energy nexus, is receiving renewed focus. The desalination industry alleviates water stress by producing freshwater from saline sources, such as seawater, brackish or groundwater. Since the last decade, the market has been dominated by membrane desalination technology, offering significant advantages over thermal processes, such as lower energy demand, easy process control and scale-up, modularity for flexible productivity, and feasibility of synergic integration of different membrane operations. Although seawater reverse osmosis (SWRO) accounts for more than 70% of the global desalination capacity, it is circumscribed by some significant technological limitations, such as: (i) the relatively low water recovery factor (around 50%) due to the negative impact of osmotic and polarization phenomena; (ii) an energy consumption in the range of 3–5 kWh m−3, still far from the theoretical energy demand (1.1 kWh m−3) to produce potable water from seawater (at 50% water recovery factor). Ultimately, desalination is an energy intensive practice and research efforts are oriented toward the development of alternative and more energy-efficient approaches in order to enhance freshwater resources without placing excessive strain on limited energy supplies. Recent years have seen a relevant surge of interest in membrane distillation (MD), a thermally driven membrane desalination technology having the potential to complement SWRO in the logic of Process Intensification and Zero Liquid Discharge paradigm. Due to its peculiar transport mechanism and negligibility of osmotic phenomena, MD allows high-quality distillate production (theoretically, non-volatile species are completely rejected) with a recovery factor of up to 80% at a relatively low operative temperature (typically 60 °C–80 °C). Although low operative temperatures make MD technology attractive for renewable power applications (e.g. solar thermal, wind or geothermal energy sources) or for efficient exploitation of low-grade or waste heat streams, the low energy efficiency intrinsically due to heat losses—and specifically to temperature polarization—has so far hindered the application at industrial scale. Nowadays, photothermal materials able to absorb and convert natural or artificial irradiation into heat have gained great attention, demonstrating the potential to mitigate the 'anthropic' energy input to MD and to mitigate the impact of thermal inefficiencies. On this road, a step-change improvement in light-to-heat conversion is expected through high-throughput computational screening over thermoplasmonic materials based on electronic and optical properties of advanced materials including novel topological phases of matter used as nanofillers in polymeric membranes. Coherently with the concept of Circular Economy, waste hypersaline solutions rejected from desalination process (referred as 'brine') are now the subject of valorization activities along two main exploitation routes: (1) recovery of valuable minor and trace metals and minerals, with special focus on critical raw materials (including, among others, Mg, Na, Ca, K, Sr, Li, Br, B, and Rb); (2) production of salinity gradient power (SGP) renewable energy resulting from the recovery of the Gibbs energy of mixing (mainly represented by the entropic contribution) of two solutions having different ionic concentration. The exciting new frontier of sustainable mining of seawater concentrates is accelerating the appearance of a plethora of innovative membrane materials and methods for brine dehydration and selective extraction of trace ions, although under the sword of Damocles represented by cost feasibility for reliable commercial application. On the other hand, among several emerging technologies, reverse electrodialysis (SGP-RED) was already proven capable—at least at the kW scale–of turning the chemical potential difference between river water, brackish water, and seawater into electrical energy. Efforts to develop a next generation of ion exchange membranes exhibiting high perm-selectivity (especially toward monovalent ions) and low electrical resistance, to improve system engineering and to optimize operational conditions, pursue the goal of enhancing the low power density so far achievable (in the order of a few W per m2). This Roadmap takes the form of a series of short contributions written independently by worldwide experts in the topic. Collectively, such contributions provide a comprehensive picture of the current state of the art in membrane science and technology at the water-energy nexus, and how it is expected to develop in the future. In addition, this Roadmap acknowledges the challenges and advances in membrane systems, particularly emphasizing the interplay of material innovation and system optimization, which collectively contribute to advancing the desalination field within the water-energy nexus framework.

A new species of the amphipod genus Pontocrates (Boeck 1871), family Oedicerotidae, is described from Cyprus in the south-eastern Mediterranean Sea. It is a sister taxon to Pontocrates moorei (Myers & Ashelby 2022), currently recorded solely from the British Isles. It is the third species of Pontocrates now known to occur in the Mediterranean Sea.

The transition to seawater desalination integrated with resource recovery, particularly in water- and energy-scarce regions, requires innovative approaches that consider societal benefits and costs. This study goes beyond traditional techno-economic evaluations by employing a Value-Sensitive Design (VSD) approach, which guides the selection of performance indicators and informs the design of technical scenarios for integrated seawater desalination and brine treatment systems. VSD ensures that the scenarios are socially relevant by directly incorporating stakeholder values into the design and assessment process. Four technical scenarios (Sc) were used to evaluate the VSD approach: Sc1) maximum water recovery, Sc2) and Sc3) integrated desalination with brine treatment for maximum resource recovery (using different configurations) and Sc4) electricity-based desalination for chemical recovery. Techno-economic models are implemented using Python to analyse the feasibility and performance of these scenarios. The modelling results indicate that all scenarios achieve zero brine production. However, the trade-offs between resource recovery and greenhouse gas emissions are evident. Increased salt recovery leads to higher CO2 emissions (locally) due to electricity consumption. Scenario 1 minimized electrical energy consumption and emissions while maximizing water production. Scenarios 2 and 3 performed best in water and high-quality salt production. Despite its higher CO2 emissions, Scenario 4 proved most profitable due to the production of chemicals. These findings highlight the importance of tailoring plant designs to regional needs. By providing a comprehensive understanding of trade-offs, the VSD approach fosters stakeholder dialogue and serves as a valuable decision-making tool for designing sustainable desalination systems.

Circular economy has become a popular subject, attracting attention from academics, practitioners, and policy-makers alike. However, despite the excitement surrounding it, the concept of circular economy has been criticized for being vague and having multiple interpretations from different fields. As a result, there is a lack of evidence and guidance for practitioners, making it difficult to put into practice. Our goal is to fill this gap by bridging the design and implementation of circular economy solutions in the water sector. Through an exploratory study of two case studies, we have shown the significance of what we call as “circular water value” in the context of coal mining. This value is strongly influenced by the chemistry, concentration levels and purity of these effluents. We compared the circular value of the two cases (ranging from 2.5 to 6 euros per cubic meter) to the cost of the novel treatment system, developed by the authors through the EU-funded project ZERO BRINE, to capture this value. This allowed us to evaluate the potential for circular economy implementation. We suggest that this circular transition can offer significant opportunities to coal mining regions in enabling a just transition implementation. This is a topic that is increasingly gaining interest among academic and practitioner communities, further triggered by the recently adopted Just Transition Mechanism. This mechanism secures targeted support of 55 billion euro for the period 2021–2027 for the most affected regions within Europe. The concept of “circular water value” introduced in this article can serve as a tool for exploring the creation of emerging circular value chains from coal mines, as well as for other wastewater treatment and resource recovery projects in general.

Water scarcity in arid regions has driven the spread of desalination. These systems contribute to water access but come at an intensive energy cost, and lead to brine discharge and associated environmental impacts. This work aims to investigate emerging societal issues and tensions when developing and implementing a thermal desalination system to produce irrigation water in the South of Spain. This has been done in a demonstration system for solar desalination able to recover water and salts from desalination brine. For this purpose, a context-sensitive design exercise has been implemented. First, tensions between social values expressed by diverse stakeholders have been identified. Then, a set of technical scenarios for the full-scale implementation of the system were designed and evaluated, comparing them to conventional membrane desalination. The analysis indicates high economic and energy costs to avoid the environmental impacts of increasing water production.

Industrial wastewater often has high levels of salt, either due to seawater or e.g. sodium chloride (NaCl) usage in the processing. Previous work indicated that aerobic granular sludge (AGS) is differently affected by seawater or saline water at similar osmotic strength. Here we investigate in more detail the impact of NaCl concentrations and seawater on the granulation and conversion processes for AGS wastewater treatment. Glycerol was used as the carbon source since it is regularly present in industrial wastewaters, and to allow the evaluation of microbial interactions that better reflect real conditions. Long-term experiments were performed to evaluate and compare the effect of salinity on granulation, anaerobic conversions, phosphate removal, and the microbial community. Smooth and stable granules as well as enhanced biological phosphorus removal (EBPR) were achieved up to 20 g/L NaCl or when using seawater. However, at NaCl levels comparable to seawater strength (30 g/L) incomplete anaerobic glycerol uptake and aerobic phosphate uptake were observed, the effluent turbidity increased, and filamentous granules began to appear. The latter is likely due to the direct aerobic growth on the leftover substrate after the anaerobic feeding period. In all reactor conditions, except the reactor with 30 g/L NaCl, Ca. Accumulibacter was the dominant microorganism. In the reactor with 30 g/L NaCl, the relative abundance of Ca. Accumulibacter decreased to ≤1 % and an increase in the genus Zoogloea was observed. Throughout all reactor conditions, Tessaracoccus and Micropruina, both actinobacteria, were present which were likely responsible for the anaerobic conversion of glycerol into volatile fatty acids. None of the glycerol metabolizing proteins were detected in Ca. Accumulibacter which supports previous findings that glycerol can not be directly utilized by Ca. Accumulibacter. The proteome profile of the dominant taxa was analysed and the results are further discussed. The exposure of salt-adapted biomass to hypo-osmotic conditions led to significant trehalose and PO₄³⁻-P release which can be related to the osmoregulation of the cells. Overall, this study provides insights into the effect of salt on the operation and stability of the EBPR and AGS processes. The findings suggest that maintaining a balanced cation ratio is likely to be more important for the operational stability of EBPR and AGS systems than absolute salt concentrations.

This work proposes an innovative integration of Membrane Distillation (MD) and photo-oxidation for a continuous recovery of water from arsenic (As) contaminated solutions coupled with the oxidation of arsenite (As(III)) into arsenate (As(V)). Polyvinylidene fluoride (PVDF) mixed matrix membranes (MMMs) containing titanium dioxide nanoparticles (TiO₂ NPs) as photocatalyst were developed. A systematic study elucidated the effect of TiO₂ NPs on membranes’ morphology prepared via non-solvent-induced phase separation (NIPS) using triethyl phosphate (TEP) as a green solvent for PVDF solubilization. Vacuum membrane distillation (VMD) tests carried out by irradiating the MMMs with ultraviolet (UV) radiation demonstrated the possibility of recovering up to 80 % of the water from As-contaminated synthetic and real multi-ions aqueous solutions from Sila Massif (Italy). The distillate was recovered at a rate of 6.9–7.2 kg·m⁻²·h⁻¹ (feed inlet temperature of 60 °C), while the presence of 7 wt% of TiO₂ in PVDF membranes enabled the photo-oxidation of 95 % of the As(III) to As(V) at a first order kinetic constant of 0.0106 min⁻¹. After 5 cycles of As-remediation experiments, post-hoc mechanical testing on the membrane suggested the emergence of polymer embrittlement induced by UV radiation (total irradiation time of 7.5 h), highlighting the urgent need for developing photocatalytic membranes with long-term stability. Overall, this study elucidates at laboratory scale the performance of a coupled and continuous Membrane Distillation (MD) and photo-oxidation system for arsenic (As) remediation, employing microporous hydrophobic green membranes doped with a photocatalyst.

Minimum Liquid Discharge (MLD) and Zero Liquid Discharge (ZLD) schemes have been widely proposed in the recent scientific literature not only as a possible solution to brine disposal but also as a non-conventional sustainable source of raw materials. Nevertheless, very few works have pushed the idea towards a real demonstration activity, and this somehow limits the reliability that such schemes have with respect to the real implementation potential at the industrial scale. In this work, for the first time in the literature, an integrated treatment chain for the sustainable production of freshwater and minerals has been demonstrated at a pre-industrial scale, in the island of Lampedusa (Italy). The treatment chain included a Nanofiltration (NF) step to separate monovalent and bivalent ions, followed by a Multi-Effect Distillation (MED) unit, powered by waste heat from a Thermal Power plant, generating high-quality water (<30 μS/cm) and an ultra-concentrated brine. The latter was treated in Evaporation Ponds (EPs) to generate high purity NaCl (>99 %). On the other side, the NF retentate was treated to selectively recover magnesium and calcium hydroxides (Mg(OH)₂ purity up to 98 %) in a novel Multiple Feed-Plug Flow Reactor (MF-PFR). The resulting brine fed an ElectroDialysis with Bipolar Membranes unit (EDBM), generating in-situ alkaline and acidic solutions: chemicals needed for internal usage in the plant. All units were successfully tested, reaching satisfactory performance indicators. Furthermore, the stability of each unit during the daily operational run was assessed and successfully achieved, demonstrating not only the technical feasibility of the proposed demo plant, but also the feasibility of MLD as a sustainable alternative for minerals recovery.

The sustainable supply of water is crucial, especially on islands where water is scarce. Our study applied the social life cycle assessment (S-LCA), under the organizational approach, to assess industrial water production on the island of Lampedusa, Italy. A novel plant for industrial water production considering a circular concept was compared with the existing linear production plant based on reverse osmosis. An online survey, brief literature review and generic analysis were conducted to prioritize impact subcategories selection for site-specific analysis that regarded six organizations in the system boundaries. These subcategories were Local employment, Access to material resources, Promoting social responsibility, End-of-life responsibility, Health and safety (Workers), and Public commitment to sustainability issues. The social performance of organizations involved was assessed based on equal weighting and weighting with cost values. The generic analysis showed that wastewater treatment in Italy is underdeveloped, and water scarcity can become a serious problem in the future. The site-specific analysis based on equal weighting showed that the novel water plant results in improving social performance for all considered impact subcategories by 88 % to 91 % due to co-production when compared with the existing plant. Even increasing impacts allocation to industrial water production social benefits are still expected due to co-production. The type of weighting based on cost values showed that two organizations are the main contributors to the social performance of the novel system, and improving their corporate conduct can result in improving impacts up to 25 %, such as Public commitment to sustainability issues. To conclude, the novel plan does provide social benefits but mainly due to co-production, thus, it should be investigated more how to apply the S-LCA to linear production systems as they become more circular.

Enhanced biological phosphate removal and aerobic sludge granulation are commonly studied with fatty acids as substrate. Fermentative substrates such as glucose have received limited attention. In this work, glucose conversion by aerobic granular sludge and its impact on phosphate removal was studied. Long-term stable phosphate removal and successful granulation were achieved. Glucose was rapidly taken up (273 mg/gVSS/h) at the start of the anaerobic phase, while phosphate was released during the full anaerobic phase. Some lactate was produced during glucose consumption, which was anaerobically consumed once glucose was depleted. The phosphate release appeared to be directly proportional to the uptake of lactate. The ratio of phosphorus released to glucose carbon taken up over the full anaerobic phase was 0.25 Pmol/Cmol. Along with glucose and lactate uptake in the anaerobic phase, poly‑hydroxy-alkanoates and glycogen storage were observed. There was a linear correlation between glucose consumption and lactate formation. While lactate accounted for approximately 89 % of the observed products in the bulk liquid, minor quantities of formate (5 %), propionate (4 %), and acetate (3 %) were also detected (mass fraction). Formate was not consumed anaerobically. Quantitative fluorescence in-situ hybridization (qFISH) revealed that polyphosphate accumulating organisms (PAO) accounted for 61 ± 15 % of the total biovolume. Metagenome evaluation of the biomass indicated a high abundance of Micropruina and Ca. Accumulibacter in the system, which was in accordance with the microscopic observations and the protein mass fraction from metaproteome analysis. Anaerobic conversions were evaluated based on theoretical ATP balances to provide the substrate distribution amongst the dominant genera. This research shows that aerobic granular sludge technology can be applied to glucose-containing effluents and that glucose is a suitable substrate for achieving phosphate removal. The results also show that for fermentable substrates a microbial community consisting of fermentative organisms and PAO develop.

The diversity and distribution of polychaetes in the coastal area and the EEZ of the Republic of Cyprus is presented based on both the literature records and new data acquired in a wide range of environmental monitoring programmes and research projects. A total of 585 polychaete species belonging to 49 families were reported in Cyprus waters; among them, 205 species (34%) were recorded based on the literature only, 149 (26%) were new records based on our own data, and a total of 231 spp. (40%) were recorded from both the literature and new data. A total of 51 polychaete species were identified as non-indigenous; among them, 32 were confirmed as alien species, 4 were considered cryptogenic, and 15 were considered questionable as there were doubts about their identity. The Indo-Pacific Schistomeringos loveni was reported for the first time in the Mediterranean Sea, while four species already reported in the literature, namely, Bispira melanostigma, Fimbriosthenelais longipinnis Leonnates aylaoberi, and Rhodopsis pusilla, were added to the list of non-indigenous polychaetes in the Mediterranean Sea. The current work highlights the importance of implementing environmental monitoring programmes and carrying out research surveys targeting benthic macrofauna assemblages.

This work explores resource recovery coupled to seawater desalination in small islands. As small islands depend on seawater desalination for water access, they make an excellent ground for exploring the trade-offs associated to resource recovery, like potential economic gains, energy use, and environmental impacts. Here, we investigated these tensions in the context of Lampedusa, in Italy. We then developed and evaluated scenarios for the recovery of additional water, Mg, and other resources from brines, to identify if and how resource recovery is an interesting approach for the island vis-à-vis these tensions. We have found that the potential to increase water production with water recovery from brine is an interesting alternative for small islands, especially when harnessing waste heat. However, while some technologies offer possibilities for recovering additional resources, in places like small islands the potential benefits from additional recovery do not seem to justify the costs to the local system.

The recovery of resources, including water reuse, has been presented as a solution to overcome scarcity, and improve the economic and environmental performance of water provision and treatment. However, its implementation faces non-technical challenges, including the need to collaborate with new stakeholders and face societal acceptance issues. Looking at the prominence of the circular economy in current policy developments and the challenges to resource recovery, exploring these issues is urgently needed. In this work, we reviewed a broad range of literature to identify societal values relevant to the recovery of water and other resources from wastewaters, particularly urban and industrial wastewater and desalination brines. We discuss tensions and uncertainties around these values, such as the tension between socio-economic expectations of resource recovery and potential long-term sustainability impacts, as well as uncertainties regarding safety and regulations. For addressing these tensions and uncertainties, we suggest aligning common methods in engineering and the natural sciences with Responsible Innovation approaches, such as Value Sensitive Design and Safe-by-Design. To complement Responsible Innovation, social learning with a Sustainability Transitions or Adaptive Governance perspective is suggested.

Glycerol is abundantly present in wastewater from industries such as biodiesel production facilities. Glycerol is also a potential carbon source for microbes that are involved in wastewater nutrient removal processes. The conversion of glycerol in biological phosphorus removal of aerobic granular sludge processes has not been explored to date. The current study describes glycerol utilization by aerobic granular sludge and enhanced biological phosphorus removal (EBPR). Robust granules with good phosphorus removal capabilities were formed in an aerobic granular sludge sequencing batch reactor fed with glycerol. The interaction between the fermentative conversion of glycerol and product uptake by polyphosphate accumulating organisms (PAO) was studied using stoichiometric and microbial community analysis. Metagenomic, metaproteomic and microscopic analysis identified a community dominated by Actinobacteria (Tessaracoccus and Micropruina) and a typical PAO known as Ca. Accumulibacter. Glycerol uptake facilitator (glpF) and glycerol kinase (glpK), two proteins involved in the transport of glycerol into the cellular metabolism, were only observed in the genome of the Actinobacteria. The anaerobic conversion appeared to be a combination of a substrate fermentation and product uptake-type reaction. Initially, glycerol fermentation led mainly to the production of 1,3-propanediol (1,3-PDO) which was not taken up under anaerobic conditions. Despite the aerobic conversion of 1,3-PDO stable granulation was observed. Over time, 1,3-PDO production decreased and complete anaerobic COD uptake was observed. The results demonstrate that glycerol-containing wastewater can effectively be treated by the aerobic granular sludge process and that fermentative and polyphosphate accumulating organisms can form a food chain in glycerol-based EBPR processes.

Eighty percent of wastewater is left untreated or not reused, exacerbating the water quality challenge, especially in vulnerable communities. This Voices asks: how can we improve wastewater management and convert wastewater into a resource?